Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights

doi:10.1016/j.jchromb.2020.122255

	Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights
	Li, Ke 1,2,3; Li, Shuying 2,3,4; Du, Yuguang 1; Qin, Xuemei 2,3
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2020-09-01
卷号	1152 页码:13
关键词	Astragalus polysaccharides for injection Innate and adaptive immunity Proliferation Phagocytic Structural analysis
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2020.122255
英文摘要	As a special traditional Chinese medicine, Astragalus polysaccharides for injection (APS, batch no. Zhunzi Z20040086) includes complex polysaccharide macromolecules that may increase the risk upon application. Although fingerprints for quality control are available, the specific active ingredients are unclear. Identifying the active components is the key to reduce the risk of adverse reactions of the drug. In this work, APS was mainly separated into two components, namely, macromolecular component (APS-I) and small molecular components (APS-II). The molecular weight measurement revealed that the average molecular weight of APS-I exceeded 500 kDa, and that of APS-II was 10 kDa. Monosaccharide-composition analysis revealed that APS-I consisted of glucose, galactose, arabinose, rhamnose, and galacturonic acid, with a ratio of approximately 1.5:1:5.4:0.08:0.1. Meanwhile, APS-II consisted of glucose, galactose, arabinose, rhamnose, and galacturonic acid, with a molar ratio of 9:1:1.4:0.04:0.001. Methylation, FT-IR, and NMR analysis indicated that the APS-I monosaccharide residue was linked as follows: D-Glcp-(1 ->, -> 4)-D-Glcp-(1 ->, -> 2)-L-Rhap-(1 ->, D-Araf-(1 ->, -> 5)-D-Araf-(1 ->, -> 2,5)-D-Araf-(1 ->, -> 4)-D-Galp-(1 -> . Meanwhile, the APS-II monosaccharide residue was connected as follows: alpha-D-Glcp-(1 ->,-> 4)-alpha-D-Glcp-(1 ->, -> 6)-alpha-D-Glcp-(1 ->, -> 4,6)-alpha-D-Glcp-(1 ->, -> 3,4,6)-alpha-D-Glcp-(1 ->, -> 2)-alpha-L-Rhap-(1 ->, alpha-D-Araf-(1 ->, -> 5)-alpha-D-Araf-(1 ->, -> 4)-beta-D-Galp-(1 -> . Screening experiments on their in vitro immunological activity showed that APS-II had stronger effect on innate and adaptive immunities than APS-I. In vivo animal experiments showed that APS-II can increase the leukocyte level of cyclophosphamide immunosuppressed mice and improve their immunomodulatory ability. Therefore, APS-II is the main active ingredient of APS and is expected to become a new generation of APS products.
资助项目	National Natural Science Foundation of China[81872962] ; National Key R&D Program of China[2019YFC1710800] ; China Postdoctoral Science Foundation Project[2019M650851] ; Science and Technology Research Project of Shanxi Province (China)[2014ZD0401] ; Key Projects of Key Research and Development Plan in Shanxi (China)[201603D311101] ; Shanxi Province Technology Innovation Project of Excellent Talent (China)[201605D211030] ; Shanxi Province Technology Innovation Project of Excellent Talent (China)[201705D211020]
WOS关键词	IMMUNOMODULATORY ACTIVITY ; ANTIOXIDANT ACTIVITIES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000566904300023
资助机构	National Natural Science Foundation of China ; National Key R&D Program of China ; China Postdoctoral Science Foundation Project ; Science and Technology Research Project of Shanxi Province (China) ; Key Projects of Key Research and Development Plan in Shanxi (China) ; Shanxi Province Technology Innovation Project of Excellent Talent (China)
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/42025]
专题	中国科学院过程工程研究所
通讯作者	Du, Yuguang; Qin, Xuemei
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 2.Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan 030006, Peoples R China 3.Shanxi Univ, Engn Minist, Key Lab Chem Biol & Mol, Taiyuan 030006, Peoples R China 4.Shanxi Univ, Coll Chem & Chem Engn, Taiyuan 030006, Peoples R China
推荐引用方式 GB/T 7714	Li, Ke,Li, Shuying,Du, Yuguang,et al. Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2020,1152:13.
APA	Li, Ke,Li, Shuying,Du, Yuguang,&Qin, Xuemei.(2020).Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1152,13.
MLA	Li, Ke,et al."Screening and structure study of active components of Astragalus polysaccharide for injection based on different molecular weights".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1152(2020):13.