Engineered hepatitis B core virus-like particle carrier for precise and personalized Alzheimer's disease vaccine preparation via fixed-point coupling

doi:10.1016/j.apmt.2020.100575

	Engineered hepatitis B core virus-like particle carrier for precise and personalized Alzheimer's disease vaccine preparation via fixed-point coupling
	Ji, Mei 1,2; Xie, Xi-xiu 2; Liu, Dong-qun 1,2; Lu, Shuai 2; Zhang, Ling-xiao 1,2; Huang, Ya-ru 1,2; Liu, Rui-tian 2
刊名	APPLIED MATERIALS TODAY
	2020-06-01
卷号	19 页码:16
关键词	Alzheimer's disease Virus-like particles (VLPs) B cell epitope peptide SpyCatcher/SpyTag Th2-type immune response
ISSN号	2352-9407
DOI	10.1016/j.apmt.2020.100575
英文摘要	Heterogenous aggregates of amyloid beta (A beta) and tau protein play a key role in Alzheimer's disease (AD) progression. As the epitope profiles of A beta and abnormally phosphorylated tau change over the course of the disease, tailor immunotherapy to specific patient groups according to the disease stage by targeting corresponding A beta and tau species or both of tau and A beta might improve treatment efficacy. However, epitope peptides have low immunogenicity and peptide vaccine preparation is laborious and time-consuming. We here first constructed a platform for peptide vaccine preparation by inserting SpyCatcher into the major immunodominant region (MIR) of truncated Hepatitis B core protein (HBc)(1-149). The resulted recombinant protein HBc-SpyCatcher (HBc-S) could assemble into uniform and stable virus-like particles (VLPs), and readily bind to the SpyTag conjugated epitope peptides. Several series of peptides such as linear, cyclic and phosphorylated peptides including A beta(1-6), A beta(1-15), cA beta(1-7), cEP1, cEP2 from (beta-amyloid monomer or oligomers, T294, pTau396-404, pTau422 from tau proteins, were glued onto HBc-S VLPs, forming HBc-S-peptide (HBc-S-P) VLPs and efficiently elicited specific Th2-type immune responses. When applied to AD transgenic mice, HBc-S-pTau422 alleviated cognition deficits and neuropathology progression in Tau.P301S transgenic mice. The present study provides an easy, quick, convenient and universal platform for high-throughput peptide epitope screening and personalized peptide vaccine preparation. (C) 2020 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[81371208] ; National Natural Science Foundation of China[81971073] ; National Natural Science Foundation of China[81903531] ; International Partnership Program of Chinese Academy of Sciences[122111KYSB20180005]
WOS关键词	A-BETA IMMUNOTHERAPY ; PEPTIDE VACCINE ; IMMUNE-RESPONSE ; AMYLOID-BETA ; MOUSE MODEL ; TAU ; DELIVERY ; ANTIBODY ; PHOSPHORYLATION ; IMMUNOGENICITY
WOS研究方向	Materials Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000546198000001
资助机构	National Natural Science Foundation of China ; International Partnership Program of Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/41360]
专题	中国科学院过程工程研究所
通讯作者	Liu, Rui-tian
作者单位	1.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Ji, Mei,Xie, Xi-xiu,Liu, Dong-qun,et al. Engineered hepatitis B core virus-like particle carrier for precise and personalized Alzheimer's disease vaccine preparation via fixed-point coupling[J]. APPLIED MATERIALS TODAY,2020,19:16.
APA	Ji, Mei.,Xie, Xi-xiu.,Liu, Dong-qun.,Lu, Shuai.,Zhang, Ling-xiao.,...&Liu, Rui-tian.(2020).Engineered hepatitis B core virus-like particle carrier for precise and personalized Alzheimer's disease vaccine preparation via fixed-point coupling.APPLIED MATERIALS TODAY,19,16.
MLA	Ji, Mei,et al."Engineered hepatitis B core virus-like particle carrier for precise and personalized Alzheimer's disease vaccine preparation via fixed-point coupling".APPLIED MATERIALS TODAY 19(2020):16.