Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates Onto Electrospun Film

doi:10.1089/ten.tea.2019.0318

	Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates Onto Electrospun Film
	Zhang, Jiabin 1; Yun, Seonho 1; Du, Yuguang 2; Zannettino, Andrew C. W.3; Zhang, Hu 1,4
刊名	TISSUE ENGINEERING PART A
	2020-03-19
页码	9
关键词	hydrogel chondrogenesis human mesenchymal stem stromal cell electrospinning cell aggregates cartilage regeneration
ISSN号	1937-3341
DOI	10.1089/ten.tea.2019.0318
英文摘要	Irregular defects at sites of degenerative cartilage often accompany osteoarthritis (OA). The development of novel cell-/biomaterial-based cartilage tissue engineering methods to address these defects may provide a durable approach to hinder the development of OA. In this study, we fabricated a neocartilage patch by fusing cell aggregates onto a biodegradable nanofiber film for degenerative cartilage repair. Human mesenchymal stem/stromal cell (MSC) aggregates were prepared and induced for chondrogenesis in a thermosensitive hydrogel, poly (N-isopropylacrylamide-co-acrylic acid (p(NIPAAm-AA)). Cell migration mediated the formation of cell aggregates in the thermosensitive hydrogel and led to a cell-dense hollow shell structure. The chondrocytes derived from MSC aggregates in the hydrogel were evidenced by the expression of chondrogenesis-related genes and extracellular matrices. They were fused onto an electrospun film by mechanical force and spatial confinement to generate a neo-cartilage patch. The fabricated neocartilage patches may be able to integrate into the irregular defects under compressive stresses and achieve cartilage regeneration in vivo. Impact statement The formation of human mesenchymal stem/stromal cells aggregates in thermosensitive hydrogels was mechanistically examined. These in situ formed cell aggregates with enhanced chondrogenesis were bioengineered into a neocartilage patch for regeneration of superficial irregular cartilage defects.
资助项目	Australian Research Council Discovery Project[DP160104632] ; Medical Advancement Without Animal (MAWA) Trust
WOS关键词	STEM-CELLS ; ARTICULAR-CARTILAGE ; TISSUE ; CULTURE ; REPAIR ; DIFFERENTIATION ; MIGRATION
WOS研究方向	Cell Biology ; Engineering ; Materials Science
语种	英语
出版者	MARY ANN LIEBERT, INC
WOS记录号	WOS:000525891200001
资助机构	Australian Research Council Discovery Project ; Medical Advancement Without Animal (MAWA) Trust
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/40085]
专题	中国科学院过程工程研究所
通讯作者	Zannettino, Andrew C. W.; Zhang, Hu
作者单位	1.Univ Adelaide, Sch Chem Engn & Adv Mat, Adelaide, SA, Australia 2.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China 3.Univ Adelaide, Adelaide Med Sch, Adelaide, SA 5001, Australia 4.Keck Grad Inst, Henry E Riggs Sch Appl Life Sci, Claremont, CA 91711 USA
推荐引用方式 GB/T 7714	Zhang, Jiabin,Yun, Seonho,Du, Yuguang,et al. Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates Onto Electrospun Film[J]. TISSUE ENGINEERING PART A,2020:9.
APA	Zhang, Jiabin,Yun, Seonho,Du, Yuguang,Zannettino, Andrew C. W.,&Zhang, Hu.(2020).Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates Onto Electrospun Film.TISSUE ENGINEERING PART A,9.
MLA	Zhang, Jiabin,et al."Fabrication of a Cartilage Patch by Fusing Hydrogel-Derived Cell Aggregates Onto Electrospun Film".TISSUE ENGINEERING PART A (2020):9.