Succinate Production with Metabolically Engineered Escherichia coli Using Elephant Grass Stalk (Pennisetum purpureum) Hydrolysate as Carbon Source

doi:10.1007/s12649-018-0524-2

	Succinate Production with Metabolically Engineered Escherichia coli Using Elephant Grass Stalk (Pennisetum purpureum) Hydrolysate as Carbon Source
	Olajuyin, Ayobami Matthew 1,2; Yang, Maohua 1,2; Mu, Tingzhen 1,2; Sharshar, Moustafa Mohamed 1,2; Xing, Jianmin 1,2
刊名	WASTE AND BIOMASS VALORIZATION
	2020-05-01
卷号	11 期号:5 页码:1717-1725
关键词	Escherichia coli Succinic acid Elephant grass stalk Dual-phase fermentation
ISSN号	1877-2641
DOI	10.1007/s12649-018-0524-2
英文摘要	Succinic acid is a spectacular chemical that can be used as the precursor of various industrial products including pharmaceuticals and biochemicals. The improvement of the succinic acid market depends on strains engineering that is capable of producing succinic acid at high yield and excellent growth rate which could utilize the wide range of carbon sources such as renewable biomass. Here we use counter selection using catAsacB for pathway design and strains developments. In this investigation, metabolically engineered Escherichia coli M6PM strain was constructed for the synthesis of succinic acid using elephant grass stalk (Pennisetum purpureum) as a carbon source. Elephant grass stalk hydrolysate was prepared which comprised of 11.60 +/- 0.04 g/L glucose, 27.22 +/- 0.04 g/L xylose and 0.65 +/- 0.04 g/L arabinose. Metabolically engineered E. coli M6PM was constructed and fermentation with pure sugars revealed that it could utilize xylose and glucose efficiently. E. coli M6PM produced a final succinate concentration of 30.03 +/- 0.02 g/L and a yield of 1.09 mol/mol during 72 h dual-phase fermentation using elephant grass stalk hydrolysate, which resulted in 64% maximum theoretical yield of succinic acid. The high succinate yield from elephant grass stalk demonstrated possible application of renewable biomass as feedstock for the synthesis of succinic acid using recombinant E. coli.
WOS关键词	EFFICIENT PRODUCTION ; ACID PRODUCTION ; PATHWAYS ; GROWTH ; ISOMERASE ; STRAINS ; YIELD ; STATE
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000523383800008
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/39952]
专题	中国科学院过程工程研究所
通讯作者	Xing, Jianmin
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Olajuyin, Ayobami Matthew,Yang, Maohua,Mu, Tingzhen,et al. Succinate Production with Metabolically Engineered Escherichia coli Using Elephant Grass Stalk (Pennisetum purpureum) Hydrolysate as Carbon Source[J]. WASTE AND BIOMASS VALORIZATION,2020,11(5):1717-1725.
APA	Olajuyin, Ayobami Matthew,Yang, Maohua,Mu, Tingzhen,Sharshar, Moustafa Mohamed,&Xing, Jianmin.(2020).Succinate Production with Metabolically Engineered Escherichia coli Using Elephant Grass Stalk (Pennisetum purpureum) Hydrolysate as Carbon Source.WASTE AND BIOMASS VALORIZATION,11(5),1717-1725.
MLA	Olajuyin, Ayobami Matthew,et al."Succinate Production with Metabolically Engineered Escherichia coli Using Elephant Grass Stalk (Pennisetum purpureum) Hydrolysate as Carbon Source".WASTE AND BIOMASS VALORIZATION 11.5(2020):1717-1725.