Quantitative profiling of integrin alpha v beta 3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma

doi:10.1039/c9nr01105f

	Quantitative profiling of integrin alpha v beta 3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma
	Wang, Tingting 2,4; Li, Guang 5; Wang, Dianbing 4; Li, Feng 1; Men, Dong 1; Hu, Tao 3; Xi, Yan 1,4; Zhang, Xian-En 2,4
刊名	NANOSCALE
	2019-10-21
卷号	11 期号:39 页码:18224-18231
ISSN号	2040-3364
DOI	10.1039/c9nr01105f
英文摘要	The distribution, localization and density of individual molecules (e.g. drug-specific receptors) on single cells can offer profound information about cell phenotypes. Profiling this information is a new research direction within the field of single cell biology, but it remains technically challenging. Through the combined use of quantum dot labeling, structured illumination microscopy (SIM) and computer-aided local surface reconstruction, we acquired a 3D imaging map of a drug target molecule, integrin alpha v beta 3, on glioblastoma cells at the single cell level. The results revealed that integrin alpha v beta 3 exhibits discrete distribution on the surface of glioblastoma cells, with its density differing significantly among cell lines. The density is illustrated as the approximate number of target molecules per mu m(2) on the irregular cell surface, ranging from 0 to 1.6. Functional studies revealed that the sensitivity of glioblastoma cells to inhibitor molecules depends on the density of the target molecules. After inhibitor treatment, the viability and invasion ability of different glioblastoma cells were highly correlated with the density of integrin alpha v beta 3 on their surfaces. This study not only provides a novel protocol for the quantitative analysis of surface proteins from irregular single cells, but also offers a clue for understanding the heterogeneity of tumor cells on the basis of molecular phenotypes. Thus, this work has potential significance in guiding targeted therapies for cancers.
资助项目	Chinese Academy of Sciences[XDB29050100] ; National Key Research and Development Program of China[2017YFA0205500]
WOS关键词	EXPRESSION ; ALPHA(V)BETA(3) ; INFORMATICS
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000512634500030
资助机构	Chinese Academy of Sciences ; National Key Research and Development Program of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/39367]
专题	中国科学院过程工程研究所
通讯作者	Wang, Dianbing; Zhang, Xian-En
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 4300071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Natl Lab Biomacromol, CAS Ctr Excellence Biomacromol, Inst Biophys, Beijing 100101, Peoples R China 5.Huazhong Agr Univ, Coll Informat, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Wang, Tingting,Li, Guang,Wang, Dianbing,et al. Quantitative profiling of integrin alpha v beta 3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma[J]. NANOSCALE,2019,11(39):18224-18231.
APA	Wang, Tingting.,Li, Guang.,Wang, Dianbing.,Li, Feng.,Men, Dong.,...&Zhang, Xian-En.(2019).Quantitative profiling of integrin alpha v beta 3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma.NANOSCALE,11(39),18224-18231.
MLA	Wang, Tingting,et al."Quantitative profiling of integrin alpha v beta 3 on single cells with quantum dot labeling to reveal the phenotypic heterogeneity of glioblastoma".NANOSCALE 11.39(2019):18224-18231.