Packaging and delivering enzymes by amorphous metal-organic frameworks

doi:10.1038/s41467-019-13153-x

	Packaging and delivering enzymes by amorphous metal-organic frameworks
	Wu, Xiaoling 10; Yue, Hua 11; Zhang, Yuanyu 9; Gao, Xiaoyong 11; Li, Xiaoyang 9; Wang, Licheng 9; Cao, Yufei 9; Hou, Miao 9; An, Haixia 11; Zhang, Lin 2,12
刊名	NATURE COMMUNICATIONS
	2019-11-14
卷号	10 页码:8
ISSN号	2041-1723
DOI	10.1038/s41467-019-13153-x
英文摘要	Enzymatic catalysis in living cells enables the in-situ detection of cellular metabolites in single cells, which could contribute to early diagnosis of diseases. In this study, enzyme is packaged in amorphous metal-organic frameworks (MOFs) via a one-pot co-precipitation process under ambient conditions, exhibiting 5-20 times higher apparent activity than when the enzyme is encapsulated in corresponding crystalline MOFs. Molecular simulation and cryo-electron tomography (Cryo-ET) combined with other techniques demonstrate that the mesopores generated in this disordered and fuzzy structure endow the packaged enzyme with high enzyme activity. The highly active glucose oxidase delivered by the amorphous MOF nanoparticles allows the noninvasive and facile measurement of glucose in single living cells, which can be used to distinguish between cancerous and normal cells.
资助项目	National Key Research and Development Plan of China[2016YFA0204300] ; National Key Research and Development Plan of China[2017YFA0403801] ; National Natural Science Foundation of China[21622603] ; National Natural Science Foundation of China[21622608] ; National Natural Science Foundation of China[21878174] ; National Natural Science Foundation of China[51573085] ; National Natural Science Foundation of China[21908070] ; National Natural Science Foundation of China[U1732120] ; Beijing Natural Science Foundation[JQ18006] ; China Postdoctoral Science Foundation[BX20180102]
WOS关键词	X-RAY ; CANCER ; AMORPHIZATION ; PLATFORM
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000496409800013
资助机构	National Key Research and Development Plan of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation ; China Postdoctoral Science Foundation
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/39160]
专题	中国科学院过程工程研究所
通讯作者	Zhang, Lin; Li, Sai; Wei, Wei; Ge, Jun
作者单位	1.Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China 2.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Syst Bioengn, Tianjin 300354, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Minist Educ, Key Lab Prot Sci, Beijing 100084, Peoples R China 4.Beijing Adv Innovat Ctr Struct Biol, Beijing 100084, Peoples R China 5.Tsinghua Shenzhen Int Grad Sch, Biopharmaceut & Hlth Engn Div, Shenzhen, Peoples R China 6.Fudan Univ, Dept Chem, Jiangwan Campus, Shanghai 200438, Peoples R China 7.Jilin Univ, State Key Lab Superhard Mat, Changchun 130012, Jilin, Peoples R China 8.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Zhangjiang Lab, Shanghai 201204, Peoples R China 9.Tsinghua Univ, Dept Chem Engn, Key Lab Ind Biocatalysis, Minist Educ, Beijing 100084, Peoples R China 10.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wu, Xiaoling,Yue, Hua,Zhang, Yuanyu,et al. Packaging and delivering enzymes by amorphous metal-organic frameworks[J]. NATURE COMMUNICATIONS,2019,10:8.
APA	Wu, Xiaoling.,Yue, Hua.,Zhang, Yuanyu.,Gao, Xiaoyong.,Li, Xiaoyang.,...&Ge, Jun.(2019).Packaging and delivering enzymes by amorphous metal-organic frameworks.NATURE COMMUNICATIONS,10,8.
MLA	Wu, Xiaoling,et al."Packaging and delivering enzymes by amorphous metal-organic frameworks".NATURE COMMUNICATIONS 10(2019):8.