Responses of rhizosphere and bulk substrate microbiome to wastewater-borne sulfonamides in constructed wetlands with different plant species

	Responses of rhizosphere and bulk substrate microbiome to wastewater-borne sulfonamides in constructed wetlands with different plant species
	Man, Ying; Wang, Jiaxi; Tam, Nora Fung-yee; Wan, Xiang; Huang, Wenda; Zheng, Yu; Tang, Jinpeng; Tao, Ran; Yang, Yang
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2020
卷号	706
英文摘要	Constructed wetlands (CWs) have been used to remove organic pollutants including antibiotics based on the roles of plants and microbial communities, but how rhizosphere and bulk substrate-associated microbiomes respond to antibiotics during biodegradation have seldom been researched. The effects of sulfonamides (SAs) on the microbiome composition in different compartments, namely rhizosphere, near rhizosphere and bulk substrate, in CWs planted with either Cyperus alternifolius, Cyperus papyrus orJuncus effuses were evaluated using specially designed rhizoboxes and 16S rRNA gene high-throughput sequencing. Results revealed that wastewater-borne SAs significantly reduced the microbial biodiversity in CWs, and inhibited the functional bacterial groups related to sulphur and nitrogen cycles. On the contrary, SAs significantly enriched methylotrophs with potential to initially biodegrade SAs, such as Methylosinus, Meihyloienera, Meihyloculdum and Methylumuncis, and such enrichment was more significant in rhizosphere than in bulk substrate. The network analysis indicated that a more complex network in bulk substrate was more fragile to SA stress. The presence of wetland plants significantly influenced the bacterial community structure in CWs, but in the same compartment, the difference among the three plants species was not obvious. Wetland plants ensured the stability of rhizosphere microorganisms and increased their ability to tolerate SA stress. The present study enhances our understanding of the importance of plant-bacteria interactions in CWs and responses of substrate microbiome to antibiotics. (C) 2019 Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://159.226.73.51/handle/332005/20238]
专题	中国科学院南京地理与湖泊研究所
推荐引用方式 GB/T 7714	Man, Ying,Wang, Jiaxi,Tam, Nora Fung-yee,et al. Responses of rhizosphere and bulk substrate microbiome to wastewater-borne sulfonamides in constructed wetlands with different plant species[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,706.
APA	Man, Ying.,Wang, Jiaxi.,Tam, Nora Fung-yee.,Wan, Xiang.,Huang, Wenda.,...&Yang, Yang.(2020).Responses of rhizosphere and bulk substrate microbiome to wastewater-borne sulfonamides in constructed wetlands with different plant species.SCIENCE OF THE TOTAL ENVIRONMENT,706.
MLA	Man, Ying,et al."Responses of rhizosphere and bulk substrate microbiome to wastewater-borne sulfonamides in constructed wetlands with different plant species".SCIENCE OF THE TOTAL ENVIRONMENT 706(2020).