Aquiflexum lacus sp. nov., isolated from a lake sediment sample

doi:10.1007/s00203-021-02280-z

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	Aquiflexum lacus sp. nov., isolated from a lake sediment sample
	Huang, Jianrong; Han, Mingxian; Fang, Baozhu; Yang, Jian; Xiao, Haiyi; Zhang, Xiying; Han, Jibin; Yu, Dongmei; Jiang, Hongchen; Li, Wenjun
刊名	ARCHIVES OF MICROBIOLOGY
	2021-08
卷号	203 期号:6 页码:2911-2917
ISSN号	0302-8933
DOI	10.1007/s00203-021-02280-z
英文摘要	A novel Gram-staining negative, crescent-like or rod-shaped, non-motile bacterium, designated strain CUG 91378( T), was isolated from a sediment sample of Qinghai Lake, Qinghai Province, China. The strain was red-colored, and catalase- and oxidase-positive. Strain CUG 91378( T) was able to grow at 15-37 degrees C (optimum, 28 degrees C), pH 7-9 (pH 7.0) and in the presence of up to 3.0% (w/v) NaCl (0-2%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CUG 91378( T) formed a well-supported monophyletic clade with Aquiflexum balticum DSM 16537( T) (95.4%) and Aquiflexum aquatile Z0201(T) (93.2%). The DNA G + C content of CUG 91378( T) was 39.0%. Low (< 87%) average nucleotide identity (ANI) and (< 26%) digital DNA-DNA hybridization (dDDH) values were observed between strain CUG 91378( T) and its closest species on the phylogenetic trees. The sole respiratory quinone of strain CUG 91378( T) was MK-7. The predominant fatty acids (> 5.0%) were iso-C-15:0 (19.1%), iso-C-16:0 (12.0%), iso-C-16:1 H (10.9%), iso-C-16:0 3OH (9.2%), iso-C-17:0 3OH (7.7%), C-17:1 omega 6c (6.1%) and anteiso-C-15:0 (5.8%). Strain CUG 91378( T) contained as phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and four unidentified lipids (L1, L2, L3 and L4). Based on the data from the current polyphasic study, the isolate represents a novel species of the genus Aquiflexum for which the name Aquiflexum lacus is proposed. The type strain of the proposed new taxon is CUG 91378( T) (= KCTC 62637( T) = CGMCC 1.13988( T)).
内容类型	期刊论文
源URL	[http://ir.isl.ac.cn/handle/363002/34223]
专题	青海盐湖研究所_盐湖地质与环境实验室
推荐引用方式 GB/T 7714	Huang, Jianrong,Han, Mingxian,Fang, Baozhu,et al. Aquiflexum lacus sp. nov., isolated from a lake sediment sample[J]. ARCHIVES OF MICROBIOLOGY,2021,203(6):2911-2917.
APA	Huang, Jianrong.,Han, Mingxian.,Fang, Baozhu.,Yang, Jian.,Xiao, Haiyi.,...&Li, Wenjun.(2021).Aquiflexum lacus sp. nov., isolated from a lake sediment sample.ARCHIVES OF MICROBIOLOGY,203(6),2911-2917.
MLA	Huang, Jianrong,et al."Aquiflexum lacus sp. nov., isolated from a lake sediment sample".ARCHIVES OF MICROBIOLOGY 203.6(2021):2911-2917.