Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines

doi:10.1016/j.carbpol.2021.118867

	Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines
	Liu, Jie 2; Wang, Hong-da 2; Yang, Fei-fei 2; Chen, Bo-xue 2; Li, Xue 2; Huang, Qing-xia 3; Li, Jing 3; Li, Xiang-yan 3; Li, Zheng 1,2; Yu, He-shui 1,2
刊名	CARBOHYDRATE POLYMERS
	2022-02-01
卷号	277 页码:10
关键词	Panax Polysaccharide Oligosaccharide spectrum Untargeted metabolomics Cardiomyocyte protection
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2021.118867
通讯作者	Guo, De-an(daguo@simm.ac.cn) ; Yang, Wen-zhi(wzyang0504@tjutcm.edu.cn)
英文摘要	The role of polysaccharides in quality control of ginseng is underestimated. Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var. major (ZZS), was performed by both chemical and biological approaches. Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function. Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively. RG polysaccharides (25/50/100 mu g/mL) showed significant cardiomyocyte protection by regulating mitochondrial functions. These new evidences may provide support for the supplementary role of polysaccharides in quality control of ginseng.
资助项目	National Natural Science Foundation of China[81872996] ; National Natural Science Foundation of China[82130111] ; Natural Science Foundation of Tianjin of China[20JCYBJC00060] ; Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine[ZYYCXTD-D-202002]
WOS关键词	PANAX-GINSENG ; METABOLISM
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000720837300001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/298982]
专题	中国科学院上海药物研究所
通讯作者	Guo, De-an; Yang, Wen-zhi
作者单位	1.Tianjin Univ Tradit Chinese Med, Coll Pharmaceut Engn Tradit Chinese Med, Tianjin 301617, Peoples R China 2.Tianjin Univ Tradit Chinese Med, State Key Lab Component Based Chinese Med, Tianjin Key Lab TCM Chem & Anal, 10 Poyanghu Rd, Tianjin 301617, Peoples R China 3.Changchun Univ Chinese Med, Jilin Ginseng Acad, Changchun 130117, Peoples R China 4.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, 501 Haike Rd, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jie,Wang, Hong-da,Yang, Fei-fei,et al. Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines[J]. CARBOHYDRATE POLYMERS,2022,277:10.
APA	Liu, Jie.,Wang, Hong-da.,Yang, Fei-fei.,Chen, Bo-xue.,Li, Xue.,...&Yang, Wen-zhi.(2022).Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines.CARBOHYDRATE POLYMERS,277,10.
MLA	Liu, Jie,et al."Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines".CARBOHYDRATE POLYMERS 277(2022):10.