Cocktail polysaccharides isolated from Ecklonia kurome against the SARS-CoV-2 infection

doi:10.1016/j.carbpol.2021.118779

	Cocktail polysaccharides isolated from Ecklonia kurome against the SARS-CoV-2 infection
	Zhang, Shihai 1,3; Pei, Rongjuan 6; Li, Meixia 3,4; Su, Haixia 4,5; Sun, Hao 6; Ding, Yaqi 3,4; Su, Minbo 4,5; Huang, Chunfan 2,3; Chen, Xia 3,4; Du, Zhenyun 3,4
刊名	CARBOHYDRATE POLYMERS
	2022
卷号	275 页码:8
关键词	COVID-19 SARS-CoV-2 Angiotensin converting enzyme 2 (ACE2) 3CL protease Ecklonia kurome Okam Polysaccharide
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2021.118779
通讯作者	Xu, Yechun(ycxu@simm.ac.cn) ; Chen, Xinwen(chenxw@wh.iov.cn) ; Zhang, Bo(zhangbo@wh.iov.cn) ; Ding, Kan(dingkan@simm.ac.cn)
英文摘要	Previous researches suggested that polysaccharides from brown algae had anti-virus activity. We hypothesized that nature polysaccharide from marine plants might have the effect on anti-SARS-CoV-2 activity. By high throughput screening to target 3CLpro enzyme using polysaccharides library, we discover a crude polysaccharide 375 from seaweed Ecklonia kurome blocked 3CLpro enzymatic activity and shows good anti-SARS-CoV-2 infection activity in cell. Further, we show that homogeneous polysaccharide 37502 from the 375 may bind to 3CLpro well and disturb spike protein binding to ACE2 receptor. The structure characterization uncovers that 37502 is alginate. These results imply that the bioactivities of 375 on SARS-CoV-2 may target multiple key molecules implicated in the virus infection and replication. The above results suggest that 375 may be a potential drug candidate against SARS-CoV-2.
资助项目	National Key Research and Development Program of China ; Ministry of Science and Technology of the People's Republic of China[2019YFC1711-000] ; Key New Drug Creation and Manufacturing Program[2019ZX09735001] ; COVID-19 Emergency Research Project by Zhejiang University[2020XGZX080] ; Shanghai Municipal Science and Technology Commission Major Project
WOS关键词	ALGINATE ; SARS ; ACID
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000712466000011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/298756]
专题	中国科学院上海药物研究所
通讯作者	Xu, Yechun; Chen, Xinwen; Zhang, Bo; Ding, Kan
作者单位	1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, 138 Xianlin Ave, Nanjing 210023, Peoples R China 2.Nanjing Univ Chinese Med, Coll Pharm, 138 Xianlin Ave, Nanjing 210023, Jiangsu, Peoples R China 3.Glycochem & Glycobiol Lab, Key Lab Receptor Res, Nanjing, Peoples R China 4.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, State Key Lab Drug Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 5.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 6.Chinese Acad Sci, Ctr Biosafety Megasci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Shihai,Pei, Rongjuan,Li, Meixia,et al. Cocktail polysaccharides isolated from Ecklonia kurome against the SARS-CoV-2 infection[J]. CARBOHYDRATE POLYMERS,2022,275:8.
APA	Zhang, Shihai.,Pei, Rongjuan.,Li, Meixia.,Su, Haixia.,Sun, Hao.,...&Ding, Kan.(2022).Cocktail polysaccharides isolated from Ecklonia kurome against the SARS-CoV-2 infection.CARBOHYDRATE POLYMERS,275,8.
MLA	Zhang, Shihai,et al."Cocktail polysaccharides isolated from Ecklonia kurome against the SARS-CoV-2 infection".CARBOHYDRATE POLYMERS 275(2022):8.