trans Single-Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction

doi:10.1002/anie.202110384

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	trans Single-Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction
	Wei, Yangdao 4; Yang, Zhiqing 4; Zong, Chengli 4; Wang, Buhua 4; Ge, Xiaolin 4; Tan, Xiao 3; Liu, Xin 1; Tao, Zhenzhen 4; Wang, Peng 2; Ma, Chunxin 4
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2021-10-04
页码	8
关键词	CRISPR-Cas14a1 DNA cleavage pathogenic bacteria RNA RNA detection platform
ISSN号	1433-7851
DOI	10.1002/anie.202110384
通讯作者	Wan, Yi(993602@hainanu.edu.cn) ; Li, Jinghong(jhli@mail.tsinghua.edu.cn)
英文摘要	As a CRISPR-Cas system (clustered regularly interspaced short palindromic repeats and CRISPR associated proteins), Cas14a1 can cis/trans cleave single-stranded DNA (ssDNA). Here, we describe an unreported capacity of Cas14a1: RNA can trigger the trans ssDNA cleavage. This Cas14a1-based RNA-activated detection platform (Amplification, Transcription, Cas14a1-based RNA-activated trans ssDNA cleavage, ATCas-RNA) has an outstanding specificity for the detection of target RNAs with point mutation resolution, which is better than that of the Cas14a1-based ssDNA-activation. Using ATCas-RNA via a fluorophore quencher-labeled ssDNA reporter (FQ), we were able to detect 1 aM pathogenic nucleic acid within 1 h, and achieve 100 % accuracy with 25 milk samples. This platform can serve as a new tool for high-efficiency nucleic acid diagnostics. Importantly, this work can expand our understanding of Cas14a1 and inspire further mechanisms and applications of Class-2 Cas systems.
资助项目	National Key Research and Development Program of China[2018YFD0900704] ; National Natural Science Foundation of China[21621003] ; National Natural Science Foundation of China[41866002] ; Tsinghua University Initiative Scientific Research Program[2020Z99CFZ019] ; Tsinghua University Spring Breeze Fund[2020Z99CFZ019] ; Research Foundation of Hainan University[KYQD(ZR)1711] ; Research Foundation of Hainan University[KYQD(ZR)1997] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF04]
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000703187000001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/176479]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Wan, Yi; Li, Jinghong
作者单位	1.Univ Shiga Prefecture, Sch Environm Sci, Dept Ecosyst Studies, 2500 Hassaka Cho, Hikone, Shiga 5228533, Japan 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 3.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China 4.Hainan Univ, Sch Life Sci, Sch Pharmaceut Sci, Marine Coll,State Key Lab Marine Resource Utiliza, Haikou 570228, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Wei, Yangdao,Yang, Zhiqing,Zong, Chengli,et al. trans Single-Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2021:8.
APA	Wei, Yangdao.,Yang, Zhiqing.,Zong, Chengli.,Wang, Buhua.,Ge, Xiaolin.,...&Li, Jinghong.(2021).trans Single-Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,8.
MLA	Wei, Yangdao,et al."trans Single-Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021):8.