Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum

doi:10.1016/j.algal.2021.102567

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum
	Liu, Xuehua 1,2,3,4; Wang, Lijun 1,2,3; Wu, Songcui 1,2,3; Zhou, Lu 1,2,3,4; Gao, Shan 1,2,3; Xie, Xiujun 1,2,3; Wang, Lepu 1,2,3,4; Gu, Wenhui 1,2,3; Wang, Guangce 1,2,3
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2022
卷号	61 页码:10
关键词	Ferritin Morphological change Oval Abscisic acid Dormancy
ISSN号	2211-9264
DOI	10.1016/j.algal.2021.102567
通讯作者	Gu, Wenhui(wenhuigu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要	The formation of resting cells/spores in marine diatoms helps them to survive in environmental changes, whose sedimentation characteristics also drive enhanced carbon fluxes export to the deep ocean. However, the molecular mechanism underlying the transformation remains unclear. Based on the upregulation of ferritin during dormancy in microorganism and its' crucial role in coping with environmental stress, we constructed the ferritin overexpression and silence strains in model marine diatom, Phaeodactylum tricornutum (FTN-OE and FTN-Si, respectively). The morphological transformation from fusiform to ovoid were observed among the FTN-OE strains but not the wide type and FTN-Si strains. These FTN-OE oval cells exhibited typical characteristics of resting cells and benthic adaptation, such as lower growth rates, low-light adaptation, silicified valves, the excretion of exopolymeric substances, upregulation of stress-resistant proteins and increased nitrate reserves. Furthermore, FTN-OE oval cells showed increased abscisic acid synthesis and signaling pathways in the transcriptome and proteome. And the abscisic acid signal was detected in FTN-OE oval cells exclusively (2.96 ng g(-1) FW), but absent in the FTN-Si, FTN-OE fusiform or WT cells. Overall, our results demonstrate that ferritin overexpression in marine diatom have a close relationship with morphological transformation from fusiform to ovoid morphotype, after which they enter a resting stage, which is accompanied by abscisic acid accumulation. This improves our understanding of the molecular processes in diatom resting cells/spores' formation and suggests that ferritin accumulation might improve the sequestration of photosynthetically fixed atmospheric CO2 into the deep sea.
资助项目	National Key R&D Program of China[2018YFD0901500] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q02] ; Shandong Provincial RD Program[2018GHY115019] ; Chinese Academy of Sciences[KLEMB-DYS02] ; National Natural Science Foundation of China[41676157] ; National Natural Science Foundation of China[41976097] ; National Natural Science Foundation of China[41806171] ; National Natural Science Foundation of China[41876158] ; Haikou Marine Economic Demonstration City Industrial Chain Collaborative Innovation Project[HHCL201811] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316]
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000730984400005
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177539]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Gu, Wenhui; Wang, Guangce
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xuehua,Wang, Lijun,Wu, Songcui,et al. Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,61:10.
APA	Liu, Xuehua.,Wang, Lijun.,Wu, Songcui.,Zhou, Lu.,Gao, Shan.,...&Wang, Guangce.(2022).Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,61,10.
MLA	Liu, Xuehua,et al."Formation of resting cells is accompanied with enrichment of ferritin in marine diatom Phaeodactylum tricornutum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 61(2022):10.