Screening and characterization of proteases produced by deep-sea cold seep bacteria

doi:10.1007/s00343-021-0441-2

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Screening and characterization of proteases produced by deep-sea cold seep bacteria
	Guo, Chenchen 2; Sun, Chaomin 1; Wu, Shimei 2
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2021-12-08
页码	12
关键词	deep-sea Bacillus alkaline protease purification characterization
ISSN号	2096-5508
DOI	10.1007/s00343-021-0441-2
通讯作者	Wu, Shimei(shimeiwu2016@126.com)
英文摘要	Fifty protease-producing strains were screened from sediment of deep-sea cold seep, and divided into four different categories: Bacillus, Pseudoalteromonas, Vibrio, and Alteromonas according to the sequences of 16s rRNA. Their abilities to produce protease, amylase, and lipase were determined, and a Bacillus strain gcc-1 displayed very strong alkaline protease activity and stability under different thermal and acidic conditions. The purification of the protease produced by strain gcc-1 was carried out by precipitation with ammonium sulfate, and sequentially chromatographed by anion exchange column and gel filtration. The purified protease showed a single band at the molecular weight of 28 kDa by SDS-PAGE. The characterization results show that the purified protease exhibited a considerable activity and stability in a wide thermal range of 10-80 degrees C and a wide acidic range of pH 6.5-11.5, and displayed highest activity at 40 degrees C and pH 8.5. Notably, the protease still maintained high activity even at low to 10 degrees C. Furthermore, the protease exhibited good stability in presence of different surfactants, organic solvents, oxidizing agent H2O2, and commercial detergents. Therefore, the protease produced by gcc-1 is a cold active and high stable enzyme, and has a promising potential in laundry detergent as an additive.
资助项目	National Natural Science Foundation of China[31600035] ; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000728779800003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177473]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wu, Shimei
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Guo, Chenchen,Sun, Chaomin,Wu, Shimei. Screening and characterization of proteases produced by deep-sea cold seep bacteria[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2021:12.
APA	Guo, Chenchen,Sun, Chaomin,&Wu, Shimei.(2021).Screening and characterization of proteases produced by deep-sea cold seep bacteria.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,12.
MLA	Guo, Chenchen,et al."Screening and characterization of proteases produced by deep-sea cold seep bacteria".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2021):12.