PUF60 of Japanese flounder is regulated by pol-miR-novel_395 and involved in pathogen infection, autophagy, and apoptosis

doi:10.1016/j.dci.2021.104170

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	PUF60 of Japanese flounder is regulated by pol-miR-novel_395 and involved in pathogen infection, autophagy, and apoptosis
	Li, Wenrui 1,2,3,4; Guan, Xiaolu 1,2,3
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2021-10-01
卷号	123 页码:9
关键词	Paralichthys olivaceus microRNA Poly (U)-Binding-splicing factor 60 kDa Pathogen infection Autophagy Apoptosis
ISSN号	0145-305X
DOI	10.1016/j.dci.2021.104170
通讯作者	Guan, Xiaolu(guanxiaolu@qdio.ac.cn)
英文摘要	MicroRNAs (miRNAs) are evolutionary conserved, non-coding small RNAs that have been shown to regulate diverse biological processes including immunity. In a previous study, a novel miRNA of Japanese flounder (Paralichthys olivaceus), pol-miR-novel_395, was found to be responsive in expression to the infection of the bacterial pathogen Edwardsiella tarda. In the present study, we examined the regulation and immune effect of polmiR-novel_395 and its target gene. We found that pol-miR-novel_395 expression was regulated by E. tarda and megalocytivirus, and pol-miR-novel_395 targeted the gene of PUF60 (poly (U)-binding-splicing factor 60 kDa) of flounder (named PoPUF60). Constitutive expression of PoPUF60 occurred in relatively high levels in the heart and liver of flounder. Bacterial infection upregulated PoPUF60 expression, whereas viral infection downregulated PoPUF60 expression. Interference with PoPUF60 expression or overexpression of pol-miR-novel_395 in flounder cells strongly potentiated E. tarda infection. Consistently, in vivo knockdown of PoPUF60 enhanced bacterial dissemination in the tissues of flounder but blocked viral replication, whereas in vivo overexpression of PoPUF60 inhibited bacterial dissemination but facilitated viral replication. Additionally, pol-miR-novel_395 and PoPUF60 were involved in the process of autophagy and apoptosis. Collectively, these results indicated that PoPUF60 and pol-miR-novel_395 play an important role in pathogen infection, autophagy, and apoptosis.
资助项目	National Natural Science Foundation of China[31730100] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ03022] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000672561000001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177014]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Guan, Xiaolu
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Wenrui,Guan, Xiaolu. PUF60 of Japanese flounder is regulated by pol-miR-novel_395 and involved in pathogen infection, autophagy, and apoptosis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,123:9.
APA	Li, Wenrui,&Guan, Xiaolu.(2021).PUF60 of Japanese flounder is regulated by pol-miR-novel_395 and involved in pathogen infection, autophagy, and apoptosis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,123,9.
MLA	Li, Wenrui,et al."PUF60 of Japanese flounder is regulated by pol-miR-novel_395 and involved in pathogen infection, autophagy, and apoptosis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 123(2021):9.