Exogenous methyl jasmonate enhances lipid production in Isochrysis galbana under nitrogen deprivation and high light

doi:10.1016/j.algal.2021.102406

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	Exogenous methyl jasmonate enhances lipid production in Isochrysis galbana under nitrogen deprivation and high light
	Cui, Hongwu 2; Yang, Feifei 3; Li, Yongfu 1,4
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2021-10-01
卷号	58 页码:8
关键词	Isochrysis galbana Methyl jasmonate Lipid Photosynthesis Nitrogen deprivation
ISSN号	2211-9264
DOI	10.1016/j.algal.2021.102406
通讯作者	Li, Yongfu(lyf_qingdao@126.com)
英文摘要	Nitrogen deprivation under high light is the most employed cultivation condition for the lipid's accumulation in microalgae. However, nitrogen deprivation causes the over-reduction of the photosystem (PS) II acceptor side in the presence of high light, confirmed by an increased V-J (the relative variable fluorescence at J-step) during cultivation. This in turn results in photo-inhibition of microalgal cells and photodamage to cellular metabolism which hinders the lipid production. To explore modifications for lipid accumulation, the exogenous methyl jasmonate (MeJA) effect was investigated on antioxidant capacity and lipid accumulation in Isochrysis galbana. Treatment with MeJA under high light nitrogen deprivation, (100 mu M concentration), significantly enhanced the activity of ribulose-1,5-bisphosphate carboxylase/oxygenase, and facilitated the electron transport in photosynthesis as indicated by increased Phi(PSII) (effective quantum yield of PSII) after MeJA treatment. The exogenous MeJA increased the antioxidant potential of related enzymes (ascorbate peroxidase and superoxide dismutase) and significantly alleviated the accumulation of reactive oxygen species (ROS) under high light nitrogen deprivation for improved cell survival and cellular metabolism in I. galbana. The improved cellular metabolism further increased the pyruvate content, which can be converted into acetyl coenzyme A (an important precursor for fatty acid synthesis). MeJA addition increased the substrate pool required for fatty acid and lipid production. Overall, exogenous MeJA enhanced lipid production in I. galbana under high light nitrogen deprivation by regulating the antioxidant capability and biosynthesis of the substrate.
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Tech-nology (Qingdao)[2018SDKJ05011] ; National Natural Science Foundation of China[32002411] ; Natural Science Foundation of the Jiangsu Higher Education Institutions of China[19KJB170010]
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000686735100019
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/176266]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Yongfu
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 3.Jiangsu Ocean Univ, Sch Ocean Engn, Lianyungang 222005, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Cui, Hongwu,Yang, Feifei,Li, Yongfu. Exogenous methyl jasmonate enhances lipid production in Isochrysis galbana under nitrogen deprivation and high light[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2021,58:8.
APA	Cui, Hongwu,Yang, Feifei,&Li, Yongfu.(2021).Exogenous methyl jasmonate enhances lipid production in Isochrysis galbana under nitrogen deprivation and high light.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,58,8.
MLA	Cui, Hongwu,et al."Exogenous methyl jasmonate enhances lipid production in Isochrysis galbana under nitrogen deprivation and high light".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 58(2021):8.