Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5

doi:10.1016/j.ijbiomac.2021.08.159

CORC > 海洋研究所 > 中国科学院海洋研究所

	Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5
	Wei, Maosheng 1,2,3,4; Geng, Lihua 1,2,3; Wang, Qingchi 1,2,3,4; Yue, Yang 1,2,3; Wang, Jing 1,2,3; Wu, Ning 1,2,3; Wang, Xiaoqing 1,2,3,4; Sun, Chaomin 1,2,3; Zhang, Quanbin 1,2,3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2021-10-31
卷号	189 页码:649-656
关键词	Exopolysaccharide Characteristics Immunostimulatory effects
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2021.08.159
通讯作者	Geng, Lihua(lhgeng@qdio.ac.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn)
英文摘要	Crude exopolysaccharides from extracellular polymeric substances produced by the marine bacterium Bacillus sp. H5 were fractionated using DEAE-Sepharose FF and Sephadex G-75 chromatography. The high molecular weight fraction (89.0 kD) from the neutral fraction was designated EPS5SH; it contained mannose, glucosamine, glucose, and galactose in a molar ratio of 1.00: 0.02: 0.07: 0.02. Infra-red, gas chromatography-mass spectrometry, electrospray ionisation-tandem mass spectrometry analysis and nuclear magnetic resonance revealed EPS5SH was a mannan with alpha-(1-* 4)-Manp, alpha-(1-* 2)-Manp, alpha-(1-* 4, 6)-Manp and 8-terminal-Manp. Preliminary in vitro experiments revealed that EPS5SH significantly upregulated nitric oxide synthesis and release of pro inflammatory factors in murine macrophage RAW264.7 cells. Western blot experiments verified the immunostimulatory effects of EPS5SH through the modulation of the NF-kappa B and MAPK signalling pathways. In conclusion, EPS5SH was a novel immunostimulatory mannan.
资助项目	China Ocean Mineral Resources RD Association[DY135-B2-14]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000702873200006
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/176529]
专题	中国科学院海洋研究所
通讯作者	Geng, Lihua; Zhang, Quanbin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS, Ctr Ocean Megasci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Ctr Ocean Megasci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wei, Maosheng,Geng, Lihua,Wang, Qingchi,et al. Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,189:649-656.
APA	Wei, Maosheng.,Geng, Lihua.,Wang, Qingchi.,Yue, Yang.,Wang, Jing.,...&Zhang, Quanbin.(2021).Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,189,649-656.
MLA	Wei, Maosheng,et al."Purification, characterization and immunostimulatory activity of a novel exopolysaccharide from Bacillus sp. H5".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 189(2021):649-656.