A macrokinetic model for myeloma cell culture based on stoichiometric balance

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	A macrokinetic model for myeloma cell culture based on stoichiometric balance
	Zhou, Feng ; Bi, Jingxiu ; Zeng, An-ping ; Yuan, Jingqi
刊名	BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
	2007-02-01
卷号	46 期号:46 页码:85-95
关键词	macrokinetic model metabolic pathways metabolic shift myeloma cell culture stoichiometric model
ISSN号	0885-4513
其他题名	Biotechnol. Appl. Biochem.
中文摘要	A macrokinetic model for a myeloma cell line is proposed. The model describes the dynamic balances of lactate, alanine, ATP and Ill during the metabolsim of glucose, glutamine and other amino acids. The metabolic pathways mainly include glycolysis, glutaminolysis, the trcicarboxylic acid cycle, the formation and utilization of amino acids, the respiratory chain, cell growth and cell death. The metabolic shift of glucose is especially considered because of a change in the rate of glycolysis. Thus the model functions in three modes to describe the behaviour of the myeloma cell line. On the basis of this model the macrokinetic bioreaction rates such as the specific substrate consumption rate, the specific growth rate, the specific acetylCoA formation rate, as well as the specific oxygen uptake rate, are estimated. The specific substrate consumption rate and the specific growth rate are then coupled into a bioreactor model such that the key variables, i.e., the cell density, the substrate and metabolite concentrations, are obtained. Experiments with batch and fed-batch cultures of a myeloma cell line (X63-Ag8.653) were used to validate the model. The prediction of the model was simulated by the rolling prediction approach.
英文摘要	A macrokinetic model for a myeloma cell line is proposed. The model describes the dynamic balances of lactate, alanine, ATP and Ill during the metabolsim of glucose, glutamine and other amino acids. The metabolic pathways mainly include glycolysis, glutaminolysis, the trcicarboxylic acid cycle, the formation and utilization of amino acids, the respiratory chain, cell growth and cell death. The metabolic shift of glucose is especially considered because of a change in the rate of glycolysis. Thus the model functions in three modes to describe the behaviour of the myeloma cell line. On the basis of this model the macrokinetic bioreaction rates such as the specific substrate consumption rate, the specific growth rate, the specific acetylCoA formation rate, as well as the specific oxygen uptake rate, are estimated. The specific substrate consumption rate and the specific growth rate are then coupled into a bioreactor model such that the key variables, i.e., the cell density, the substrate and metabolite concentrations, are obtained. Experiments with batch and fed-batch cultures of a myeloma cell line (X63-Ag8.653) were used to validate the model. The prediction of the model was simulated by the rolling prediction approach.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	INTRACELLULAR FLUX ANALYSIS ; STEADY-STATE MULTIPLICITY ; HYBRIDOMA CELLS ; ANTIBODY-PRODUCTION ; DYNAMIC-MODEL ; STRUCTURED MODEL ; MURINE HYBRIDOMA ; REACTION NETWORK ; MAMMALIAN-CELLS ; MASS BALANCES
收录类别	SCI
原文出处	://WOS:000244194600002
语种	英语
WOS记录号	WOS:000244194600002
公开日期	2013-10-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3533]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Shanghai Jiao Tong Univ, Dept Automat, Shanghai 200240, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing 200080, Peoples R China 3.German Res Ctr Biotechnol, Div Biochem Engn, D-38124 Braunschweig, Germany
推荐引用方式 GB/T 7714	Zhou, Feng,Bi, Jingxiu,Zeng, An-ping,et al. A macrokinetic model for myeloma cell culture based on stoichiometric balance[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2007,46(46):85-95.
APA	Zhou, Feng,Bi, Jingxiu,Zeng, An-ping,&Yuan, Jingqi.(2007).A macrokinetic model for myeloma cell culture based on stoichiometric balance.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,46(46),85-95.
MLA	Zhou, Feng,et al."A macrokinetic model for myeloma cell culture based on stoichiometric balance".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 46.46(2007):85-95.