Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish

doi:10.1021/acssensors.9b01717

	Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish
	Ma, Jianlong 1,2,3; Si, Tiantian 1,2,3; Yan, Chaoxian 4; Li, Yijing 1,2; Li, Qiang 5; Lu, Xiaofeng 1,2; Guo, Yong 1,2
刊名	ACS SENSORS
	2020
卷号	5 期号:1 页码:83-92
关键词	fluorescence probes near-infrared zebrafish bioimaging in situ acetylcholinesterase
ISSN号	2379-3694
DOI	10.1021/acssensors.9b01717
通讯作者	Guo, Yong(guoyong@licp.cas.cn)
英文摘要	Acetylcholinesterase (AChE) plays crucial roles in numerous physiological processes such as cell differentiation, cell apoptosis, and nerve tissue developments. Hence, it is highly necessary to design a fluorescent probe for monitoring AChE activity in complex living organisms. In this work, a near-infrared (NIR) off-on probe (CyN) was developed for AChE detection. CyN was exactly synthesized by introducing an N,N-dimethyl carbamyl moiety to hemicyanine (CyOH). AChE can "light up" strong NIR fluorescence through a cleavage special ester bond and transform CyN into CyOH. Moreover, CyN was qualified for imaging the dynamic change of AChE activity in PC12 cells with retinoic acid or hypoxia stimulation. In particular, the probe has been successfully applied for in situ tracing the intact distribution of AChE in living zebrafish. The observations indicate that major occurrence sites of endogenic AChE on zebrafish are the yolk sac and neuromasts. Overall, CyN shows great potential for use in AChE-related physiological studies.
资助项目	National Natural Science Foundation of China[21575149] ; National Natural Science Foundation of China[21505146] ; National Natural Science Foundation of China[21575148]
WOS关键词	TURN-ON ASSAY ; FLUOROMETRIC ASSAY ; HYPOCHLOROUS ACID ; PERIPHERAL SITE ; LATERAL-LINE ; EXPRESSION ; SENSOR ; BUTYRYLCHOLINESTERASE ; VISUALIZATION ; PRECURSORS
WOS研究方向	Chemistry ; Science & Technology - Other Topics
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000510079300013
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/141669]
专题	中国科学院近代物理研究所
通讯作者	Guo, Yong
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant, Lanzhou, Gansu, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Gansu, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou, Peoples R China 5.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Ma, Jianlong,Si, Tiantian,Yan, Chaoxian,et al. Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish[J]. ACS SENSORS,2020,5(1):83-92.
APA	Ma, Jianlong.,Si, Tiantian.,Yan, Chaoxian.,Li, Yijing.,Li, Qiang.,...&Guo, Yong.(2020).Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish.ACS SENSORS,5(1),83-92.
MLA	Ma, Jianlong,et al."Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish".ACS SENSORS 5.1(2020):83-92.