Depletion of protein thiols and the accumulation of oxidized thioredoxin in Parkinsonism disclosed by a red-emitting and environment-sensitive probe

doi:10.1039/c8tb03101k

	Depletion of protein thiols and the accumulation of oxidized thioredoxin in Parkinsonism disclosed by a red-emitting and environment-sensitive probe
	Hu, Guodong 1; Zhang, Baoxin 1; Zhou, Pengcheng 1; Hou, Yanan 1; Jia, Huiyi 1; Liu, Yuxin 1; Gan, Lu 2,3; Zhang, Hong 2; Mao, Yiheng 1; Fang, Jianguo 1
刊名	JOURNAL OF MATERIALS CHEMISTRY B
	2019-04-28
卷号	7 期号:16 页码:2696-2702
ISSN号	2050-750X
DOI	10.1039/c8tb03101k
通讯作者	Fang, Jianguo(fangjg@lzu.edu.cn)
英文摘要	Protein sulfhydryl groups play a vital role in maintaining cellular redox homeostasis and protein functions and have attracted increasing interests for the selective detection of protein thiols over low-molecular-weight thiols (LMWTs). Herein, we reported a red-emitting and environment-sensitive probe (FM-red) for detecting and labeling protein thiols. The probe contains a maleimide unit as a thiol receptor and an environment-sensitive fluorophore as a sensor. The emission signal of the probe was exclusively switched on by binding to protein sulfhydryl groups through the twisted intramolecular charge transfer mechanism, while negligible fluorescence was observed when FM-red reacted with LMWTs. Various experiments verified that FM-red possessed fast responsivity (B10 min) and high selectivity to sense protein thiols over LMWTs with a red emission (similar to 655 nm). These favorable properties enable the probe to image protein sulfhydryl groups in live cells and in vivo. In addition, as FM-red has a relatively high molecular weight (MW 688), it is able to separate the labeled proteins from the unlabeled ones after FM-red derivatization via routine protein electrophoresis, which may be applied to determine the redox states of thioredoxin, a small redox protein ubiquitous in all cells. With the aid of the probe, we demonstrated a significant decrease in the protein thiols and the accumulation of oxidized thioredoxin in a cellular model of Parkinson's disease.
资助项目	National Natural Science Foundation of China[21572093] ; National Natural Science Foundation of China[21778028] ; National Natural Science Foundation of China[21708017] ; Natural Science Foundation of Gansu Province[17JR5RA193] ; Lanzhou University (the Fundamental Research Funds for the Central Universities)[lzujbky-2018-64] ; 111 project
WOS关键词	FLUORESCENT-PROBES ; OXIDATIVE STRESS ; CELL-DEATH ; DESIGN STRATEGIES ; RISK-FACTOR ; HOMOCYSTEINE ; PROTECTION ; CYSTEINE ; SYSTEM ; POLYPHENOL
WOS研究方向	Materials Science
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000493915200014
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Gansu Province ; Lanzhou University (the Fundamental Research Funds for the Central Universities) ; 111 project
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/141571]
专题	中国科学院近代物理研究所
通讯作者	Fang, Jianguo
作者单位	1.Lanzhou Univ, State Key Lab Appl Organ Chem, Coll Chem & Chem Engn, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Dept Heavy Ion Radiat Med, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Hu, Guodong,Zhang, Baoxin,Zhou, Pengcheng,et al. Depletion of protein thiols and the accumulation of oxidized thioredoxin in Parkinsonism disclosed by a red-emitting and environment-sensitive probe[J]. JOURNAL OF MATERIALS CHEMISTRY B,2019,7(16):2696-2702.
APA	Hu, Guodong.,Zhang, Baoxin.,Zhou, Pengcheng.,Hou, Yanan.,Jia, Huiyi.,...&Fang, Jianguo.(2019).Depletion of protein thiols and the accumulation of oxidized thioredoxin in Parkinsonism disclosed by a red-emitting and environment-sensitive probe.JOURNAL OF MATERIALS CHEMISTRY B,7(16),2696-2702.
MLA	Hu, Guodong,et al."Depletion of protein thiols and the accumulation of oxidized thioredoxin in Parkinsonism disclosed by a red-emitting and environment-sensitive probe".JOURNAL OF MATERIALS CHEMISTRY B 7.16(2019):2696-2702.