Genetic polymorphisms in mutagenesis progeny of Arabidopsis thaliana irradiated by carbon-ion beams and gamma-rays irradiations

doi:10.1080/09553002.2020.1688412

	Genetic polymorphisms in mutagenesis progeny of Arabidopsis thaliana irradiated by carbon-ion beams and gamma-rays irradiations
	Chen, Xia 1,2; Feng, Hui 1; Du, Yan 1; Luo, Shanwei 1; Li, Wenjian 1; Yu, Lixia 1; Feng, Zhuo 1,2; Cui, Tao 1,2; Zhou, Libin 1,2,3,4
刊名	INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
	2019-11-15
页码	9
关键词	Carbon-ion beams gamma-rays Arabidopsis thaliana ISSR RAPD
ISSN号	0955-3002
DOI	10.1080/09553002.2020.1688412
通讯作者	Zhou, Libin(libinzhoulz@gmail.com)
英文摘要	Purpose: Heavy-ion beams and gamma-rays are popular physical mutagenesis to generate mutations in higher plants. It has been found that they show different mutation frequencies and spectrums of phenotype induction, however, the characteristics of heavy-ion beams on genetic polymorphism have not been clarified by comparing with gamma-rays. Materials and methods: In the present study, seeds of Arabidopsis thaliana were exposed to carbon-ion beams (with linear energy transfer (LET) of 50 keV/mu m) and gamma-rays (with average LET of 0.2 keV/mu m) irradiation. By using inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) analysis, the genetic polymorphism of both M-1 and M-3 plants were investigated, respectively. Results: Carbon-ion beams induced relatively higher polymorphism rate in both M-1 and M-3 generation than gamma-rays: the polymorphism rates of M-1 plants derived from carbon-ion beams irradiation are 12.87% (ISSR-C) and 9.01% (RAPD-C), while are 7.67% (ISSR-gamma) and 1.45% (RAPD-gamma) of plants derived from gamma-rays. In M-3 generation, the polymorphism rates of ISSR-C, RAPD-C, ISSR-gamma, and RAPD-gamma are 17.64%, 22.79%, 12.10%, and 2.82%, respectively. Conclusions: In summary, the exposure to carbon-ion beams and gamma-rays lead to the change of genomic DNA of A. thaliana, which could be tested in M-1 plants and M-3 plants by ISSR and RAPD technology. So, both carbon-ion beams and gamma-rays can induce variations of genetic polymorphisms in M-1 plants and M-3 plants. The genetic polymorphisms of M-1 plants and M-3 plants induced by carbon-ion beams are higher than gamma-rays, indicating that heavy-ion beams irradiations mutation breeding is more advantageous than conventional ionizing radiations. Average molecular polymorphism of M-1 plants is lower than M-3 mutants, by nearly 4.77% (ISSR-C), 13.78% (RAPD-C), 4.43% (ISSR-gamma), and 1.37% (RAPD-gamma). We hope our study will provide basic information for understanding the effects of carbon-ion beams and gamma-rays for plant mutation breeding.
资助项目	National Natural Science Foundation of China[11975285] ; National Natural Science Foundation of China[11705249] ; Guangdong Procincial Key Research and Development Program[2018B020206002] ; Science and Technology Research Cooperation Program of Sichuan Provincial Institutes and Universities[18SYXHZ0014]
WOS关键词	DNA POLYMORPHISM ; MOLECULAR NATURE ; MUTATION-RATE ; DIVERSITY ; MUTANT ; ACCESSIONS ; COLOR ; RAPD
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Nuclear Science & Technology ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000497011600001
资助机构	National Natural Science Foundation of China ; Guangdong Procincial Key Research and Development Program ; Science and Technology Research Cooperation Program of Sichuan Provincial Institutes and Universities
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/141568]
专题	中国科学院近代物理研究所
通讯作者	Zhou, Libin
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Dept Biophys, Biomed Ctr, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China 4.Baiyin Innovat Acad Heavy Ion Bioind, Baiyin, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xia,Feng, Hui,Du, Yan,et al. Genetic polymorphisms in mutagenesis progeny of Arabidopsis thaliana irradiated by carbon-ion beams and gamma-rays irradiations[J]. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,2019:9.
APA	Chen, Xia.,Feng, Hui.,Du, Yan.,Luo, Shanwei.,Li, Wenjian.,...&Zhou, Libin.(2019).Genetic polymorphisms in mutagenesis progeny of Arabidopsis thaliana irradiated by carbon-ion beams and gamma-rays irradiations.INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,9.
MLA	Chen, Xia,et al."Genetic polymorphisms in mutagenesis progeny of Arabidopsis thaliana irradiated by carbon-ion beams and gamma-rays irradiations".INTERNATIONAL JOURNAL OF RADIATION BIOLOGY (2019):9.