Radiosensitization of NSCLC cells to X-rays and carbon ions by the CHK1/CHK2 inhibitor AZD7762, Honokiol and Tunicamycin

doi:10.1007/s00411-020-00867-6

	Radiosensitization of NSCLC cells to X-rays and carbon ions by the CHK1/CHK2 inhibitor AZD7762, Honokiol and Tunicamycin
	Liu, Bingtao 1,2,3,4; Chen, Weiqiang 1,2,3,4; Li, Hongbin 1,2,3,4; Li, Feifei 1,2,3,4; Jin, Xiaodong 1,2,3,4; Li, Qiang 1,2,3,4
刊名	RADIATION AND ENVIRONMENTAL BIOPHYSICS
	2020-08-28
页码	10
关键词	Radiosensitivity X-rays Carbon ions AZD7762 Honokiol Tunicamycin
ISSN号	0301-634X
DOI	10.1007/s00411-020-00867-6
通讯作者	Jin, Xiaodong(jinxd@impcas.ac.cn) ; Li, Qiang(liqiang@impcas.ac.cn)
英文摘要	Although radiotherapy, especially carbon-ion radiotherapy, is an effective treatment modality against non-small-cell lung cancer (NSCLC), studies using radiation combined with sensitizer for improving the efficacy of radiotherapy are still needed. In this work, we aimed to investigate in NSCLC A549 and H1299 cell lines the effects of different linear energy transfer (LET) radiations combined with diverse sensitizing compounds. Cells pretreated with the CHK1/CHK2 inhibitor AZD7762, Honokiol or Tunicamycin were irradiated with low-LET X-rays and high-LET carbon ions. Cell survival was assessed using the clonogenic cell survival assay. Cell cycle distribution and apoptosis were measured with flow cytometry, and DNA double strand break (DSB) and repair were detected using gamma-H2AX immunofluorescence staining. Our results revealed that AZD7762, Honokiol and Tunicamycin demonstrated low cytotoxicity to NSCLC cells and a pronounced radiosensitizing effect on NSCLC cells exposed to carbon ions than X-rays. Unrepaired DNA DSB damages, the abrogation of G2/M arrest induced by irradiation, and finally apoptotic cell death were the main causes of the radiosensitizing effect. Thus, our data suggest that high-LET carbon ion combined with these compounds may be a potentially effective therapeutic strategy for locally advanced NSCLC.
资助项目	National Key Research Program of China[2016YFC0904700] ; National Key Research Program of China[2016YFC0904702] ; National Natural Science Foundation of China[11875299] ; National Natural Science Foundation of China[U1532264] ; National Natural Science Foundation of China Academy of Engineering Physics[U1730133] ; Natural Science Foundation of Gansu Province[17JRSRA310]
WOS关键词	LUNG-CANCER ; RADIOTHERAPY ; DNA ; RADIATION ; EFFICACY ; BEAM ; COMBINATION ; CHECKPOINT ; CARCINOMA ; SURVIVAL
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Biophysics ; Environmental Sciences & Ecology ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000563611700001
资助机构	National Key Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China Academy of Engineering Physics ; Natural Science Foundation of Gansu Province
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/139885]
专题	中国科学院近代物理研究所
通讯作者	Jin, Xiaodong; Li, Qiang
作者单位	1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Gansu, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Bingtao,Chen, Weiqiang,Li, Hongbin,et al. Radiosensitization of NSCLC cells to X-rays and carbon ions by the CHK1/CHK2 inhibitor AZD7762, Honokiol and Tunicamycin[J]. RADIATION AND ENVIRONMENTAL BIOPHYSICS,2020:10.
APA	Liu, Bingtao,Chen, Weiqiang,Li, Hongbin,Li, Feifei,Jin, Xiaodong,&Li, Qiang.(2020).Radiosensitization of NSCLC cells to X-rays and carbon ions by the CHK1/CHK2 inhibitor AZD7762, Honokiol and Tunicamycin.RADIATION AND ENVIRONMENTAL BIOPHYSICS,10.
MLA	Liu, Bingtao,et al."Radiosensitization of NSCLC cells to X-rays and carbon ions by the CHK1/CHK2 inhibitor AZD7762, Honokiol and Tunicamycin".RADIATION AND ENVIRONMENTAL BIOPHYSICS (2020):10.