MicroRNA-29b-3p enhances radiosensitivity through modulating WISP1-mediated mitochondrial apoptosis in prostate cancer cells

doi:10.7150/jca.48216

	MicroRNA-29b-3p enhances radiosensitivity through modulating WISP1-mediated mitochondrial apoptosis in prostate cancer cells
	Mao, Aihong 2,3; Tang, Jinzhou 2; Tang, Deping 4; Wang, Fang 3; Liao, Shiqi 2; Yuan, Hongxia 2; Tian, Caiping 2; Sun, Chao 3; Si, Jing 3; Zhang, Hong 3
刊名	JOURNAL OF CANCER
	2020
卷号	11 期号:21 页码:6356-6364
关键词	MiR-29b-3p WISP1 radiosensitivity apoptosis prostate cancer
ISSN号	1837-9664
DOI	10.7150/jca.48216
通讯作者	Zhang, Hong(zhangh@impcas.ac.cn) ; Xia, Xiaojun(xiaxiaojun2006@163.com)
英文摘要	Radiotherapy is frequently applied for clinically localized prostate cancer while its efficacy could be significantly hindered by radioresistance. MicroRNAs (miRNAs) are important regulators in mediating cellular responses to ionizing radiation (IR), and strongly associate with radiosensitivity in many cancers. In this study, enhancement of radiosensitivity by miR-29b-3p was demonstrated in prostate cancer cell line LNCaP in vitro. Results showed that miR-29b-3p expression was significantly upregulated in response to IR from both X-rays and carbon ion irradiations. Knockdown of miR-29b-3p resulted in radioresistance while overexpression of miR-29b-3p led to increased radiosensitivity (showing reduced cell viability, suppressed cell proliferation and decreased colony formation). In addition, miR-29b-3p was found to directly target Wntl-inducible-signaling protein 1 (WISP]). Inhibition of WISP] facilitated the mitochondrial apoptosis pathway through suppressing Bcl-XL expression while activating caspase-3 and poly (ADP-ribose) polymerase (PARP). The results indicated that miR-29b-3p was a radiosensitizing miRNAs and could enhance radiosensitivity of LNCaP cells by targeting WISP1. These findings suggested a novel treatment to overcome radioresistance in prostate cancer patients, especially those with higher levels of the WISP1 expression.
资助项目	National Key R&D project of China[2018YFE0205100] ; National Natural Science Foundation of China[11665003] ; Cancer Research Youth Science Foundation of Chinese Anti-Cancer Association[CAYC18A06]
WOS关键词	MIR-29B ; WISP-1 ; METASTASIS ; ACTIVATION ; EXPRESSION ; RESISTANCE
WOS研究方向	Oncology
语种	英语
出版者	IVYSPRING INT PUBL
WOS记录号	WOS:000575093300007
资助机构	National Key R&D project of China ; National Natural Science Foundation of China ; Cancer Research Youth Science Foundation of Chinese Anti-Cancer Association
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/139705]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong; Xia, Xiaojun
作者单位	1.Gansu Prov Canc Hosp, Lanzhou, Peoples R China 2.Gansu Prov Acad Inst Med Res, Lanzhou, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China 4.Lanzhou Jiaotong Univ, Sch Chem & Biol Engn, Lanzhou 730070, Peoples R China
推荐引用方式 GB/T 7714	Mao, Aihong,Tang, Jinzhou,Tang, Deping,et al. MicroRNA-29b-3p enhances radiosensitivity through modulating WISP1-mediated mitochondrial apoptosis in prostate cancer cells[J]. JOURNAL OF CANCER,2020,11(21):6356-6364.
APA	Mao, Aihong.,Tang, Jinzhou.,Tang, Deping.,Wang, Fang.,Liao, Shiqi.,...&Xia, Xiaojun.(2020).MicroRNA-29b-3p enhances radiosensitivity through modulating WISP1-mediated mitochondrial apoptosis in prostate cancer cells.JOURNAL OF CANCER,11(21),6356-6364.
MLA	Mao, Aihong,et al."MicroRNA-29b-3p enhances radiosensitivity through modulating WISP1-mediated mitochondrial apoptosis in prostate cancer cells".JOURNAL OF CANCER 11.21(2020):6356-6364.