Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice

doi:10.1038/s41420-021-00731-6

	Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice
	Zhou, Heng 2,3,4; Yang, Pengfei 2,3,4; Li, Haining 5; Zhang, Liying 6; Li, Jin 1,2,3; Zhang, Tianyi 2,3,4; Sheng, Chengyan 1; Wang, Jufang 2,3,4
刊名	CELL DEATH DISCOVERY
	2021-11-03
卷号	7 期号:1 页码:10
DOI	10.1038/s41420-021-00731-6
通讯作者	Zhou, Heng(hengzhou@impcas.ac.cn) ; Wang, Jufang(jufangwang@impcas.ac.cn)
英文摘要	Numerous studies have shown that carbon ion radiotherapy (CIRT) induces anti-cancer immune responses in melanoma patients, yet the mechanism remains elusive. The abundance of myeloid-derived suppressor cells (MDSC) in the tumour microenvironment is associated with therapeutic efficacy and disease outcome. This study analysed the changes in the immune contexture in response to the carbon ion treatment. The murine melanoma B16, MelanA, and S91 tumour models were established in syngeneic immunocompetent mice. Then, the tumours were irradiated with carbon ion beams, and flow cytometry was utilised to observe the immune contexture changes in the bone marrow, peripheral blood, spleen, and tumours. The immune infiltrates in the tumour tissues were further assessed using haematoxylin/eosin staining and immunohistochemistry. The immunoblot detected the expression of proteins associated with the JAK/STAT signalling pathway. The secretion of immune-related cytokines was examined using ELISA. Compared to conventional radiotherapy, particle beams have distinct advantages in cancer therapy. Here, the use of carbon ion beams (5 GyE) for melanoma-bearing mice was found to reduce the population of MDSC in the bone marrow, peripheral blood, and spleen of the animals via a JAK2/STAT3-dependent mechanism. The percentage of CD3(+), CD4(+), CD8(+) T cells, macrophages, and natural killer cells increased after radiation, resulting in reduced tumour growth and prolonged overall survival in the three different mouse models of melanoma. This study, therefore, substantiated that CIRT boosts anti-tumour immune responses via the inhibition of MDSC.
资助项目	Hundred-Talent Program of the Chinese Academy of Sciences[29Y763050] ; National Nature Science Foundation of China[11905264] ; Science and Technology Research Project of Gansu Province[17JR5RA307] ; Science and Technology Research Project of Gansu Province[145RTSA012]
WOS关键词	T-CELLS ; TUMOR ; RADIATION ; CANCER ; MECHANISMS
WOS研究方向	Cell Biology
语种	英语
出版者	SPRINGERNATURE
WOS记录号	WOS:000714376400003
资助机构	Hundred-Talent Program of the Chinese Academy of Sciences ; National Nature Science Foundation of China ; Science and Technology Research Project of Gansu Province
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/138326]
专题	中国科学院近代物理研究所
通讯作者	Zhou, Heng; Wang, Jufang
作者单位	1.Lanzhou Univ, Sch Nucl Sci & Technol, Lanzhou, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Key Lab Space Radiobiol Gansu Prov, Lanzhou, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Gansu Prov Canc Hosp, Gansu Prov Acad Inst Med Sci, Lanzhou, Peoples R China 6.Gansu Univ Chinese Med, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Heng,Yang, Pengfei,Li, Haining,et al. Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice[J]. CELL DEATH DISCOVERY,2021,7(1):10.
APA	Zhou, Heng.,Yang, Pengfei.,Li, Haining.,Zhang, Liying.,Li, Jin.,...&Wang, Jufang.(2021).Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice.CELL DEATH DISCOVERY,7(1),10.
MLA	Zhou, Heng,et al."Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice".CELL DEATH DISCOVERY 7.1(2021):10.