LsrB, the hub of ABC transporters involved in the membrane damage mechanisms of heavy ion irradiation in Escherichia coli

doi:10.1080/09553002.2021.1987565

	LsrB, the hub of ABC transporters involved in the membrane damage mechanisms of heavy ion irradiation in Escherichia coli
	Li, Xin 1,2,5; Chen, Lei 1; Zhou, Haitao 3; Gu, Shaobin 1; Wu, Ying 1; Wang, Bing 1; Zhang, Miaomiao 4,7; Ding, Nan 4,7; Sun, Jiaju 1; Pang, Xinyue 6
刊名	INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
	2021-10-28
页码	10
关键词	ABC transporter biofilm heavy ion protein-protein interaction (PPI) network quorum sensing (QS) transcriptomic
ISSN号	0955-3002
DOI	10.1080/09553002.2021.1987565
通讯作者	Pang, Xinyue(pangxy78@haust.edu.cn) ; Lu, Dong(ld@impcas.ac.cn)
英文摘要	Background Ionizing radiation, especially heavy ion (HI) beams, has been widely used in biology and medicine. However, the mechanism of membrane damage by such radiation remains primarily uncharacterized. Purpose Transcriptomic profiles of Escherichia coli (E. coli) treated with HI illustrated the response mechanisms of the membrane, mainly ABC transporters, related genes regulated by antibiotics treatment through enrichment analyses of GO and KEGG. The networks of protein-protein interactions indicated that LsrB was the crucial one among the ABC transporters specially regulated by HI through the calculation of plugins MCODE and cytoHubba of Cytoscape. Finally, the expression pattern, GO/KEGG enrichment terms, and the interaction between nine LuxS/AI-2 quorum sensing system members were investigated. Conclusions Above all, results suggested that HI might perform membrane damage through regulated material transport, inhibited LuxS/AI-2 system, finally impeded biofilm formation. This work provides further evidence for the role of ABC transporters, especially LsrB, in membrane damage of E. coli to HI. It will provide new strategies for improving the precise application of HI.
资助项目	National Natural Science Foundation of China[11975284] ; National Natural Science Foundation of China[11905265]
WOS关键词	BEAM IRRADIATION ; CARBON ; CELLS
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Nuclear Science & Technology ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000713921800001
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/138298]
专题	中国科学院近代物理研究所
通讯作者	Pang, Xinyue; Lu, Dong
作者单位	1.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang, Peoples R China 2.Natl Demonstrat Ctr Expt Food Proc & Safety Educ, Luoyang, Peoples R China 3.Zhengzhou Univ, Neurol Dept, Luoyang Cent Hosp, Luoyang, Peoples R China 4.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China 5.Key Lab Microbial Resources Exploitat & Utilizat, Luoyang, Peoples R China 6.Henan Univ Sci & Technol, Coll Med Technol & Engn, Luoyang, Peoples R China 7.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Xin,Chen, Lei,Zhou, Haitao,et al. LsrB, the hub of ABC transporters involved in the membrane damage mechanisms of heavy ion irradiation in Escherichia coli[J]. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,2021:10.
APA	Li, Xin.,Chen, Lei.,Zhou, Haitao.,Gu, Shaobin.,Wu, Ying.,...&Lu, Dong.(2021).LsrB, the hub of ABC transporters involved in the membrane damage mechanisms of heavy ion irradiation in Escherichia coli.INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,10.
MLA	Li, Xin,et al."LsrB, the hub of ABC transporters involved in the membrane damage mechanisms of heavy ion irradiation in Escherichia coli".INTERNATIONAL JOURNAL OF RADIATION BIOLOGY (2021):10.