Effects of Carbon Ion Beam Irradiation on Butanol Tolerance and Production of Clostridium acetobutylicum

doi:10.3389/fmicb.2020.602774

	Effects of Carbon Ion Beam Irradiation on Butanol Tolerance and Production of Clostridium acetobutylicum
	Gao, Yue 1,3; Zhang, Miaomiao 1,2,3; Zhou, Xiang 1,3; Guo, Xiaopeng 1,3; Lei, Cairong 1; Li, Wenjian 1,2,3; Lu, Dong 1,2,3
刊名	FRONTIERS IN MICROBIOLOGY
	2020-12-18
卷号	11 页码:13
关键词	Clostridium acetobutylicum carbon ion beam irradiation mutant membrane permeability response surface methodology
ISSN号	1664-302X
DOI	10.3389/fmicb.2020.602774
通讯作者	Lu, Dong(ld@impcas.ac.cn)
英文摘要	Clostridium acetobutylicum (C. acetobutylicum) has considerable potential for use in bioenergy development. Owing to the repeated use of traditional mutagenesis methods, the strains have developed a certain tolerance. The rheology of the bioprocess and the downstream processing of the product heavily depend on the ability of C. acetobutylicum mutants to produce butanol. Carbon ion beam irradiation has advantages over traditional mutation methods for fermentative production because of its dose conformity and superb biological effectiveness. However, its effects on the specific productivity of the strains have not been clearly understood. In this study, we screened five mutants through carbon ion beam irradiation; mutant Y217 achieved a butanol-production level of 13.67 g/L, exceeding that of wild-type strain ATCC 824 (i.e., 9.77 g/L). In addition, we found that the mutant maintained normal cell membrane integrity under the stimulation of 15 g/L butanol, whereas the intracellular macromolecules of wild-type strain ATCC 824 leaked significantly. Subsequently, we used the response surface methodology (RSM) to determine if the mutant cell membrane integrity improved the butanol tolerance. We verified that with the addition of butanol, the mutant could be fermented to produce 8.35 g/L butanol, and the final butanol concentration in the fermentation broth could reach 16.15 g/L. In this study, we proved that under butanol stress, mutant Y217 features excellent butanol production and tolerance and cell membrane integrity and permeability; no prior studies have attempted to do so. This will serve as an interesting and important illustration of the complexity of genetic control of the irradiation mutation of C. acetobutylicum strains. It may also prove to be useful in the bioengineering of strains of the mutant for use in the predevelopment stage.
资助项目	National Natural Science Foundation of China[11975284] ; National Natural Science Foundation of China[11905265] ; Science and Technology Service Network Initiative of Chinese Academy of Sciences[KFJ-STS-QYZD-197] ; Project of Lanzhou Science and Technology[2019-1-39]
WOS关键词	FERMENTATION PROCESS ; BEIJERINCKII MUTANT ; TWEEN 80 ; OPTIMIZATION ; ACETONE ; HYDROLYSATE ; STRAW
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000603975200001
资助机构	National Natural Science Foundation of China ; Science and Technology Service Network Initiative of Chinese Academy of Sciences ; Project of Lanzhou Science and Technology
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/138166]
专题	中国科学院近代物理研究所
通讯作者	Lu, Dong
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing, Peoples R China 2.Gansu Key Lab Microbial Resources Exploitat & App, Lanzhou, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yue,Zhang, Miaomiao,Zhou, Xiang,et al. Effects of Carbon Ion Beam Irradiation on Butanol Tolerance and Production of Clostridium acetobutylicum[J]. FRONTIERS IN MICROBIOLOGY,2020,11:13.
APA	Gao, Yue.,Zhang, Miaomiao.,Zhou, Xiang.,Guo, Xiaopeng.,Lei, Cairong.,...&Lu, Dong.(2020).Effects of Carbon Ion Beam Irradiation on Butanol Tolerance and Production of Clostridium acetobutylicum.FRONTIERS IN MICROBIOLOGY,11,13.
MLA	Gao, Yue,et al."Effects of Carbon Ion Beam Irradiation on Butanol Tolerance and Production of Clostridium acetobutylicum".FRONTIERS IN MICROBIOLOGY 11(2020):13.