CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells

doi:10.1016/j.cellsig.2021.110012

	CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells
	Liu, Bingtao 1,2,4,7; Li, Hongbin 6; Liu, Xiongxiong 1,2,4,7; Li, Feifei 5; Chen, Weiqiang 1,2,4,7; Kuang, Yanbei 1,2,4,7; Zhao, Xueshan 3; Li, Linying 1,2,4,7; Yu, Boyi 1,2,4,7; Jin, Xiaodong 1,2,4,7
刊名	CELLULAR SIGNALLING
	2021-08-01
卷号	84 页码:11
关键词	circZNF208 SNCA miR-7-5p Radio-sensitivity Non-small cell lung cancer
ISSN号	0898-6568
DOI	10.1016/j.cellsig.2021.110012
通讯作者	Jin, Xiaodong(jinxd@impcas.ac.cn) ; Li, Qiang(liqiang@impcas.ac.cn)
英文摘要	Background: Mounting evidence suggests that circular RNAs (circRNAs) are closely related to the regulation of gene expression during tumour development. However, the role of circRNAs in modulating the radiosensitivity of non-small cell lung cancer (NSCLC) cells has not been explored. Methods: Transcriptome sequencing was used to explore the expression profiles of circRNAs in NSCLC. The expression level of circRNAs was changed by inducing instantaneous knockdown or overexpression. Changes in proliferation and radiosensitivity of NSCLC cells were investigated using CCK-8, EDU, and clonal survivals. Results: By analysing the circRNA expression profile of NSCLC cells, we found that circRNA ZNF208 (circZNF208) was significantly upregulated in a radioresistant NSCLC cell line (A549-R11), which was acquired from the parental NSCLC cell line A549. Knockout experiments indicated that circZNF208 enhanced the radiosensitivity of A549 and A549-R11 cells to X-rays. Mechanistically, circZNF208 upregulated SNCA expression by acting as a sponge of miR-7-5p and subsequently promoted the resistance of NSCLC cells to low linear energy transfer (LET) X-rays. However, this effect was not observed in NSCLC cells exposed to high-LET carbon ions. Conclusions: Knockdown of circZNF208 altered the radiosensitivity of patients with NSCLC to X-rays but did not significantly change the sensitivity to carbon ions. Therefore, circZNF208 might serve as a potential biomarker and therapeutic target for NSCLC treatment with radiotherapy of different modalities.
资助项目	National Key Research Program of China[2018YFC0115700] ; National Key Research Program of China[2018YFC0115702] ; National Natural Science Foundation of China[11875299] ; National Natural Science Foundation of China[U1532264] ; Key Deployment Project of Chinese Academy of Sciences[KFZDSW222] ; National Natural Science Foundation of China Academy of Engineering Physics[U1730133] ; Natural Science Foundation of Gansu Province[17JRSRA310]
WOS关键词	CIRCULAR RNAS ; COLORECTAL-CANCER ; MICRORNA-7 ; RESISTANCE ; BIOMARKER ; CIRCRNAS ; PATHWAY ; TARGETS ; MIRNA ; IDENTIFICATION
WOS研究方向	Cell Biology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000657674900005
资助机构	National Key Research Program of China ; National Natural Science Foundation of China ; Key Deployment Project of Chinese Academy of Sciences ; National Natural Science Foundation of China Academy of Engineering Physics ; Natural Science Foundation of Gansu Province
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/137148]
专题	中国科学院近代物理研究所
通讯作者	Jin, Xiaodong; Li, Qiang
作者单位	1.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Lanzhou Univ, Lanzhou 730000, Peoples R China 4.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Gansu, Peoples R China 5.Northwest Normal Univ, Lanzhou, Gansu, Peoples R China 6.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Bingtao,Li, Hongbin,Liu, Xiongxiong,et al. CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells[J]. CELLULAR SIGNALLING,2021,84:11.
APA	Liu, Bingtao.,Li, Hongbin.,Liu, Xiongxiong.,Li, Feifei.,Chen, Weiqiang.,...&Li, Qiang.(2021).CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells.CELLULAR SIGNALLING,84,11.
MLA	Liu, Bingtao,et al."CircZNF208 enhances the sensitivity to X-rays instead of carbon-ions through the miR-7-5p /SNCA signal axis in non-small-cell lung cancer cells".CELLULAR SIGNALLING 84(2021):11.