Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii

doi:10.1111/jse.12265

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	Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii
	Xing, Jiale 2; Liu, Peng 2; Zhao, Lei ; Huang, Fang
刊名	FRONTIERS IN PLANT SCIENCE
	2017
卷号	8
关键词	C. reinhardtii x32 mutant PSII photo-oxidative stress singlet oxygen
ISSN号	1664-462X
DOI	10.1111/jse.12265
文献子类	Article
英文摘要	The green alga Chlamydomonas reinhardtii is a key model organism for studying photosynthesis and oxidative stress in unicellular eukaryotes. Using a forward genetics approach, we have identified and characterized a mutant x32, which lacks a predicted protein named CGLD1 (Conserved in Green Lineage and Diatom 1) in GreenCut2, under normal and stress conditions. We show that loss of CGLD1 resulted in minimal photoautotrophic growth and PSII activity in the organism. We observed reduced amount of PSII complex and core subunits in the x32 mutant based on blue-native (BN)/PAGE and immunoblot analysis. Moreover, x32 exhibited increased sensitivity to high-light stress and altered tolerance to different reactive oxygenic species (ROS) stress treatments, i.e., decreased resistance to H2O2/or tert-Butyl hydroperoxide (t-BOOH) and increased tolerance to neutral red (NR) and rose bengal (RB) that induce the formation of singlet oxygen, respectively. Further analysis via quantitative real-time PCR (qRT-PCR) indicated that the increased singlet-oxygen tolerance of x32 was largely correlated with up-regulated gene expression of glutathione-S-transferases (GST). The phenotypical and physiological implications revealed from our experiments highlight the important roles of CGLD1 in maintaining structure and function of PSII as well as in protection of Chlamydomonas under photo-oxidative stress conditions.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	PHOTOSYSTEM-II ; OXIDATIVE STRESS ; HIGH-LIGHT ; THYLAKOID MEMBRANE ; SYNTHETIC BIOLOGY ; SULFUR-DEPLETION ; GENE-EXPRESSION ; PROTEIN ; REPAIR ; CYANOBACTERIA
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000406115000013
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670239, 31470340] ; National Basic Research Program of China (973 Program)National Basic Research Program of China [2015CB150100] ; Chinese Academy of SciencesChinese Academy of Sciences [XDB17000000, KGCX2-YW-373]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22360]
专题	中科院光生物学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xing, Jiale,Liu, Peng,Zhao, Lei,et al. Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Xing, Jiale,Liu, Peng,Zhao, Lei,&Huang, Fang.(2017).Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii.FRONTIERS IN PLANT SCIENCE,8.
MLA	Xing, Jiale,et al."Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii".FRONTIERS IN PLANT SCIENCE 8(2017).