Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry

doi:10.11646/phytotaxa.321.2.8

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry
	Tang, Feng 2; Chen, Naizhi ; Zhao, Meiling 2; Wang, Yucheng ; He, Ruiping ; Peng, Xianjun ; Shen, Shihua
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2017
卷号	18 期号:8
关键词	WOX transcription factor paper mulberry development phytohormone stress response
DOI	10.11646/phytotaxa.321.2.8
文献子类	Article
英文摘要	The WOX (WUSCHEL-related homeobox) is a plant-specific transcription factor involved in plant development and stress response. However, few studies have been reported on the WOX gene in woody plants. In this study, 10 BpWOX genes were isolated from paper mulberry by RACE-PCR and categorized into three clades through phylogenetic analysis, ancient, intermediate and WUS clade. Among them, five members had the transcriptional activity detected by yeast one-hybrid and seven were uniquely localized to the nucleus through green fluorescent protein (GFP) observation. The expression patterns of BpWOX genes in different tissues and under diverse treatments were quantified by the qRT-PCR method. Results showed that BpWUS was expressed in the apical bud, stem and root, BpWOX5 and BpWOX7 functioned only in the root tip, and three BpWOXs regulated leaf development redundantly. BpWOX9 and BpWOX10 were induced by indole-3-acetic acid (IAA) or jasmonic acid (JA), while BpWOX2 was repressed by five phytohormones. Interestingly, most BpWOX genes were responsive to the abiotic stress stimuli of drought, salt, cold, and cadmium (CdCl2). Together, our study revealed that BpWOXs were functionally divergent during paper mulberry development and environmental adaptation, which might be related to their evolutionary relationships. Our work will benefit the systematic understanding of the precise function of WOX in plant development and environmental stress responses.
学科主题	Plant Sciences
电子版国际标准刊号	1422-0067
出版地	BASEL
WOS关键词	HOMEOBOX GENE ; ORGAN DEVELOPMENT ; ROOT DEVELOPMENT ; BLADE OUTGROWTH ; WUSCHEL GENE ; CELL FATE ; ARABIDOPSIS ; SHOOT ; LEAF ; LEAVES
语种	英语
出版者	MDPI
WOS记录号	WOS:000410684600008
资助机构	Poverty Relief Project of the Chinese Academy of Sciences [KFJ-FP-24] ; Huimin Technology Demonstration Project of the National Modern Agricultural Science and Technology Achievements City [Z151100001015008]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22346]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Tang, Feng,Chen, Naizhi,Zhao, Meiling,et al. Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(8).
APA	Tang, Feng.,Chen, Naizhi.,Zhao, Meiling.,Wang, Yucheng.,He, Ruiping.,...&Shen, Shihua.(2017).Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(8).
MLA	Tang, Feng,et al."Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.8(2017).