The Maize AP2/EREBP Transcription Factor ZmEREB160 Enhances Drought Tolerance in Arabidopsis

doi:10.1007/s12042-020-09259-y

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	The Maize AP2/EREBP Transcription Factor ZmEREB160 Enhances Drought Tolerance in Arabidopsis
	Liu, Wenping 3; Zhao, Biligen-Gaowa ; Chao, Qing 1; Wang, Baichen 1; Zhang, Qian ; Zhang, Chunxiao 3; Li, Shufang 3; Jin, Fengxue 3; Yang, Deguang ; Li, Xiaohui 3
刊名	TROPICAL PLANT BIOLOGY
	2020
卷号	13 期号:3 页码:251-261
关键词	AP2 EREBP Drought ABA Maize
ISSN号	1935-9756
DOI	10.1007/s12042-020-09259-y
文献子类	Article
英文摘要	Drought is a main factor affecting plant growth and crop production. APETALA2/Ethylene Responsive Element Binding Protein (AP2/EREBP) transcription factors (TFs) are involved in the response to abiotic stress and their functions in ABA signaling and the regulation of drought response have been intensively studied. However, AP2/EREBP TFs have been limited in the maize. The objective of our study was to identify the function of the novel maize AP2/EREBP gene ZmEREB160. Expression levels analysis in maize revealed that ZmEREB160 is significantly induced by PEG6000, NaCl and ABA treatment. ZmEREB160 localized to the nucleus when transiently expressed in Arabidopsis leaf protoplasts. ZmEREB160 activated the reporter gene and exhibited transcriptional activation activity in yeast cells. When overexpression of ZmEREB160 in Arabidopsis significantly enhanced tolerance to osmotic and ABA stress, overexpressed seedlings were longer roots under ABA and mannitol treatments compared with wild type. In addition, overexpression of ZmEREB160 Arabidopsis seedlings was found to elevate survival rate compared with wild type plants under drought stress. During the drought treatment, qRT-PCR assays showed that the expression levels of ABA/drought stress-related genes, ABI2, ABI5, COR15, DREB2A and RD29B were up-regulated in ZmEREB160 transgenic plants, transgenic plants accumulated more proline content than wild type plants. These results indicate that ZmEREB160 functions in response to drought and ABA stresses, and participates in ABA signaling pathway and may enhance drought tolerance.
学科主题	Plant Sciences
电子版国际标准刊号	1935-9764
出版地	NEW YORK
WOS关键词	DNA-BINDING PROTEINS ; AP2 DOMAIN ; GENE-EXPRESSION ; STRESS RESPONSES ; LOW-TEMPERATURE ; SALT ; THALIANA ; FAMILY ; RICE ; OVEREXPRESSION
WOS研究方向	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000526627100001
资助机构	Young Talents of Northeast Agricultural University [17QC01]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21802]
专题	中科院光生物学重点实验室
作者单位	1.Jilin Acad Agr Sci, Crop Germplasm Resources Inst, Gongzhuling 136100, Peoples R China 2.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 3.Northeast Agr Univ, Agron Coll, Harbin 150030, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wenping,Zhao, Biligen-Gaowa,Chao, Qing,et al. The Maize AP2/EREBP Transcription Factor ZmEREB160 Enhances Drought Tolerance in Arabidopsis[J]. TROPICAL PLANT BIOLOGY,2020,13(3):251-261.
APA	Liu, Wenping.,Zhao, Biligen-Gaowa.,Chao, Qing.,Wang, Baichen.,Zhang, Qian.,...&Li, Xiaohui.(2020).The Maize AP2/EREBP Transcription Factor ZmEREB160 Enhances Drought Tolerance in Arabidopsis.TROPICAL PLANT BIOLOGY,13(3),251-261.
MLA	Liu, Wenping,et al."The Maize AP2/EREBP Transcription Factor ZmEREB160 Enhances Drought Tolerance in Arabidopsis".TROPICAL PLANT BIOLOGY 13.3(2020):251-261.