Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3

doi:10.1111/jipb.12936

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	Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3
	Wang, Xuemei 1; Yang, Zhipan 1; Zhang, Yi 3; Zhou, Wen 1; Zhang, Aihong 3; Lu, Congming 3
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2020
卷号	62 期号:11 页码:1741-1761
关键词	chloroplast group II intron P-class pentatricopeptide repeat protein splicing ycf3 intron 1
ISSN号	1672-9072
DOI	10.1111/jipb.12936
文献子类	Article
英文摘要	To gain a better understanding of the molecular mechanisms of photosystem I (PSI) biogenesis, we characterized the Arabidopsis thaliana photosystem I biogenesis factor 2 (pbf2) mutant, which lacks PSI complex. PBF2 encodes a P-class pentatricopeptide repeat (PPR) protein. In the pbf2 mutants, we observed a striking decrease in the transcript level of only one gene, the chloroplast gene ycf3, which is essential for PSI assembly. Further analysis of ycf3 transcripts showed that PBF2 is specifically required for the splicing of ycf3 intron 1. Computational prediction of binding sequences and electrophoretic mobility shift assays reveal that PBF2 specifically binds to a sequence in ycf3 intron 1. Moreover, we found that PBF2 interacted with two general factors for group II intron splicing CHLOROPLAST RNA SPLICING2-ASSOCIATED FACTOR1 (CAF1) and CAF2, and facilitated the association of these two factors with ycf3 intron 1. Our results suggest that PBF2 is specifically required for the splicing of ycf3 intron 1 through cooperating with CAF1 and CAF2. Our results also suggest that additional proteins are required to contribute to the specificity of CAF-dependent group II intron splicing.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1744-7909
出版地	HOBOKEN
WOS关键词	STRUCTURAL BASIS ; RNA-BINDING ; ARABIDOPSIS ; INTRON ; TOLERANCE ; PHOTOSYNTHESIS ; BIOSYNTHESIS ; RESPONSES ; PROMOTES ; COMPLEX
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	WILEY
WOS记录号	WOS:000532713700001
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730102, 31401035] ; Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSW-SMC003]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21763]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Shandong Agr Univ, State Key Lab Crop Biol, Coll Life Sci, Tai An 271018, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xuemei,Yang, Zhipan,Zhang, Yi,et al. Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2020,62(11):1741-1761.
APA	Wang, Xuemei,Yang, Zhipan,Zhang, Yi,Zhou, Wen,Zhang, Aihong,&Lu, Congming.(2020).Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,62(11),1741-1761.
MLA	Wang, Xuemei,et al."Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 62.11(2020):1741-1761.