GbMYBR1fromGinkgo bilobarepresses phenylpropanoid biosynthesis and trichome development inArabidopsis

doi:10.1007/s00425-020-03476-1

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	GbMYBR1fromGinkgo bilobarepresses phenylpropanoid biosynthesis and trichome development inArabidopsis
	Su, Xiaojia 2,3,4; Xia, Yaying 2,3,4; Jiang, Wenbo ; Shen, Guoan 5; Pang, Yongzhen
刊名	PLANTA
	2020
卷号	252 期号:4
关键词	GbMYBR1 Ginkgo biloba Phenylpropanoids Repressor Transcriptional regulation Trichome
ISSN号	0032-0935
DOI	10.1007/s00425-020-03476-1
文献子类	Article
英文摘要	Main Conclusion GbMYBR1, a new type of R2R3-MYB repressor fromGinkgo biloba, displayed pleiotropic effects on plant growth, phenylpropanoid accumulation, by regulating multiple related genes at different levels. Ginkgo bilobais a typical gymnosperm that has been thriving on earth for millions of years. MYB transcription factors (TFs) play important roles in diverse processes in plants. However, the role of MYBs remains largely unknown inGinkgo. Here, an MYB TF gene fromGinkgo, designated asGbMYBR1, was found to act as a repressor in multiple processes.GbMYBR1was mainly expressed in the leaves ofGinkgo.Over-expression ofGbMYBR1inArabidopsis thalianaled to growth retardation, decreases in lignin content, reduced trichome density, and remarkable reduction in anthocyanin and flavonol contents in leaves.Proanthocyanidin content was decreased in the seeds of transgenicArabidopsis, which led to light-brown seed color. Both qPCR and transcriptome sequencing analyses demonstrated that the transcript levels of multiple genes related to phenylpropanoid biosynthesis, trichome formation, and pathogen resistance were down-regulated in the transgenicArabidopsis.In particular, we found that GbMYBR1 directly interacts with the bHLH cofactor GL3 as revealed by yeast two-hybrid assays. Our work indicated thatGbMYBR1has pleiotropic effects on plant growth, phenylpropanoid accumulation, and trichome development, mediated by interaction with GL3 or direct suppression of key pathway genes. Thus,GbMYBR1represents a novel type of R2R3 MYB repressor.
学科主题	Plant Sciences
电子版国际标准刊号	1432-2048
出版地	NEW YORK
WOS关键词	R2R3-MYB TRANSCRIPTION FACTOR ; ANTHOCYANIN BIOSYNTHESIS ; FLAVONOID BIOSYNTHESIS ; NEGATIVE REGULATOR ; CHEMICAL-ANALYSIS ; AUXIN TRANSPORT ; QUALITY-CONTROL ; ARABIDOPSIS ; MYB ; GENE
WOS研究方向	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000576911700001
资助机构	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31670305, 31570306] ; Fundamental Research Funds for Central Non-profit Scientific Institution [2019-YWF-YB-06] ; Central Public-Interest Scientific Institution Basal Research Fund [Y2020GH01-3]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21562]
专题	中科院北方资源植物重点实验室
作者单位	1.Inst Med Plant Dev, Beijing 100193, Peoples R China 2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China 3.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Su, Xiaojia,Xia, Yaying,Jiang, Wenbo,et al. GbMYBR1fromGinkgo bilobarepresses phenylpropanoid biosynthesis and trichome development inArabidopsis[J]. PLANTA,2020,252(4).
APA	Su, Xiaojia,Xia, Yaying,Jiang, Wenbo,Shen, Guoan,&Pang, Yongzhen.(2020).GbMYBR1fromGinkgo bilobarepresses phenylpropanoid biosynthesis and trichome development inArabidopsis.PLANTA,252(4).
MLA	Su, Xiaojia,et al."GbMYBR1fromGinkgo bilobarepresses phenylpropanoid biosynthesis and trichome development inArabidopsis".PLANTA 252.4(2020).