Cryo-EM structure of a Ca2+-bound photosynthetic LH1-RC complex containing multiple alpha beta-polypeptides

doi:10.1038/s41467-020-18748-3

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	Cryo-EM structure of a Ca2+-bound photosynthetic LH1-RC complex containing multiple alpha beta-polypeptides
	Tani, Kazutoshi ; Kanno, Ryo ; Makino, Yuki ; Hall, Malgorzata ; Takenouchi, Mizuki ; Imanishi, Michie 4; Yu, Long-Jiang ; Overmann, Joerg 6,7; Madigan, Michael T.; Kimura, Yukihiro 4
刊名	NATURE COMMUNICATIONS
	2020
卷号	11 期号:1
ISSN号	2041-1723
DOI	10.1038/s41467-020-18748-3
文献子类	Article
英文摘要	The light-harvesting-reaction center complex (LH1-RC) from the purple phototrophic bacterium Thiorhodovibrio strain 970 exhibits an LH1 absorption maximum at 960nm, the most red-shifted absorption for any bacteriochlorophyll (BChl) a-containing species. Here we present a cryo-EM structure of the strain 970 LH1-RC complex at 2.82 angstrom resolution. The LH1 forms a closed ring structure composed of sixteen pairs of the alpha beta -polypeptides. Sixteen Ca ions are present in the LH1 C-terminal domain and are coordinated by residues from the alpha beta -polypeptides that are hydrogen-bonded to BChl a. The Ca2+-facilitated hydrogen-bonding network forms the structural basis of the unusual LH1 redshift. The structure also revealed the arrangement of multiple forms of alpha- and beta -polypeptides in an individual LH1 ring. Such organization indicates a mechanism of interplay between the expression and assembly of the LH1 complex that is regulated through interactions with the RC subunits inside. Here the authors report a cryo-EM structure of the light-harvesting-reaction center complex (LH1- RC) from the purple phototrophic bacterium Thiorhodovibrio strain 970, providing insights into the mechanisms that underlie the absorbance properties of both the LH1 and the RC of this spectrally unusual purple bacterium.
学科主题	Multidisciplinary Sciences
出版地	BERLIN
WOS关键词	LIGHT-HARVESTING COMPLEXES ; Q(Y) RED-SHIFT ; CORE COMPLEX ; REACTION CENTERS ; PURPLE BACTERIA ; CALCIUM-IONS ; THERMOCHROMATIUM-TEPIDUM ; SPECTROSCOPIC PROPERTIES ; RHODOBACTER-SPHAEROIDES ; ELECTRONIC-PROPERTIES
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE RESEARCH
WOS记录号	WOS:000577121500018
资助机构	Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED [JP20am0101118, 1758, JP20am0101116, 1878, 17am0101116j0001, 18am0101116j0002, 19am0101116j0003] ; Okinawa Institute of Science and TechnologyOkinawa Institute of Science & Technology Graduate University ; Japanese Cabinet Office ; JSPS KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; Takeda Science FoundationTakeda Science Foundation (TSF) ; Kurata Memorial Hitachi Science and Technology Foundation, Japan ; National Key R&D Program of China [2019YFA0904600]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21557]
专题	中科院光生物学重点实验室
作者单位	1.Southern Illinois Univ, Dept Microbiol, Carbondale, IL 62901 USA 2.Mie Univ, Grad Sch Med, Tsu, Mie 5148507, Japan 3.Kanno, Ryo; Hall, Malgorzata; Humbel, Bruno M.] Grad Univ OIST, Okinawa Inst Sci & Technol, Res Support Div, Imaging Sect, 1919-1 Tancha, Okinawa 9040495, Japan 4.Ibaraki Univ, Fac Sci, Mito, Ibaraki 3108512, Japan 5.Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Nada Ku, Kobe, Hyogo 6578501, Japan 6.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynthesis Res Ctr, Beijing 100093, Peoples R China 7.Leibniz Inst DSMZ German Collect Microorganism, D-38124 Braunschweig, Germany 8.Braunschweig Univ Technol, Inst Microbiol, Fac Life Sci, Braunschweig, Germany
推荐引用方式 GB/T 7714	Tani, Kazutoshi,Kanno, Ryo,Makino, Yuki,et al. Cryo-EM structure of a Ca2+-bound photosynthetic LH1-RC complex containing multiple alpha beta-polypeptides[J]. NATURE COMMUNICATIONS,2020,11(1).
APA	Tani, Kazutoshi.,Kanno, Ryo.,Makino, Yuki.,Hall, Malgorzata.,Takenouchi, Mizuki.,...&Wang-Otomo, Zheng-Yu.(2020).Cryo-EM structure of a Ca2+-bound photosynthetic LH1-RC complex containing multiple alpha beta-polypeptides.NATURE COMMUNICATIONS,11(1).
MLA	Tani, Kazutoshi,et al."Cryo-EM structure of a Ca2+-bound photosynthetic LH1-RC complex containing multiple alpha beta-polypeptides".NATURE COMMUNICATIONS 11.1(2020).