Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass

doi:10.1007/s00425-018-2940-8

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	Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass
	Yu, Juanjuan 6; Zhang, Yongxue 6; Liu, Junming ; Wang, Lin ; Liu, Panpan ; Yin, Zepeng ; Guo, Siyi 1; Ma, Jun 5; Lu, Zhuang 4; Wang, Tai 4
刊名	PLANTA
	2018
卷号	248 期号:5 页码:1079-1099
关键词	Germin-like protein Hydrogen peroxide response Proteomics Puccinellia tenuiflora Roots Signaling
ISSN号	0032-0935
DOI	10.1007/s00425-018-2940-8
文献子类	Article
英文摘要	Main conclusion Hydrogen peroxide-responsive pathways in roots of alkaligrass analyzed by proteomic studies and PutGLP enhance the plant tolerance to saline-, alkali- and cadmium-induced oxidative stresses. Oxidative stress adaptation is critical for plants in response to various stress environments. The halophyte alkaligrass (Puccinellia tenuiflora) is an outstanding pasture with strong tolerance to salt and alkali stresses. In this study, iTRAQ- and 2DE-based proteomics approaches, as well as qRT-PCR and molecular genetics, were employed to investigate H2O2-responsive mechanisms in alkaligrass roots. The evaluation of membrane integrity and reactive oxygen species (ROS)-scavenging systems, as well as abundance patterns of H2O2-responsive proteins/genes indicated that Ca2+-mediated kinase signaling pathways, ROS homeostasis, osmotic modulation, and transcriptional regulation were pivotal for oxidative adaptation in alkaligrass roots. Overexpressing a P. tenuiflora germin-like protein (PutGLP) gene in Arabidopsis seedlings revealed that the apoplastic PutGLP with activities of oxalate oxidase and superoxide dismutase was predominantly expressed in roots and played important roles in ROS scavenging in response to salinity-, alkali-, and CdCl2-induced oxidative stresses. The results provide insights into the fine-tuned redox-responsive networks in halophyte roots.
学科主题	Plant Sciences
电子版国际标准刊号	1432-2048
出版地	NEW YORK
WOS关键词	HYDROGEN-PEROXIDE STRESS ; PUCCINELLIA-TENUIFLORA ; OXIDATIVE STRESS ; PLASMA-MEMBRANE ; SALT STRESS ; ARABIDOPSIS-THALIANA ; EXPRESSION ANALYSIS ; ANALYSIS REVEALS ; NITRIC-OXIDE ; CROSS-TALK
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000447030900004
资助机构	National Key Research and Development Program [2017YFD0600101] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2572017ET01, 2572017EA05, 2572016AA16] ; Foundation of Shanghai Science and Technology Committee, China [17391900600]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20723]
专题	中科院植物分子生理学重点实验室
作者单位	1.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 2.Univ Florida, Interdisciplinary Ctr Biotechnol Res, Plant Mol & Cellular Program, Genet Inst,Dept Biol, Gainesville, FL 32610 USA 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China 5.Henan Univ, Dept Biol, State Key Lab Cotton Biol, Inst Plant Stress Biol, Kaifeng 455000, Peoples R China 6.Northeast Forestry Univ, Key Lab Saline Alkali Vegetat Ecol Restorat Minis, Alkali Soil Nat Environm Sci Ctr, Harbin 150040, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Yu, Juanjuan,Zhang, Yongxue,Liu, Junming,et al. Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass[J]. PLANTA,2018,248(5):1079-1099.
APA	Yu, Juanjuan.,Zhang, Yongxue.,Liu, Junming.,Wang, Lin.,Liu, Panpan.,...&Dai, Shaojun.(2018).Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass.PLANTA,248(5),1079-1099.
MLA	Yu, Juanjuan,et al."Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass".PLANTA 248.5(2018):1079-1099.