QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F-2 Population

doi:10.1016/j.rsci.2018.04.001

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	QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F-2 Population
	Qin Yaobin ; Cheng Peng 2; Cheng Yichen ; Feng Yue ; Huang Derun ; Huang Tingxu 3; Song Xianjun 2; Ying Jiezheng
刊名	RICE SCIENCE
	2018
卷号	25 期号:3 页码:121-131
关键词	grain length grain weight QTL-seq quantitative trait locus near-isogenic line rice single nucleotide polymorphism recombinant inbred line qTGW5.3
ISSN号	1672-6308
DOI	10.1016/j.rsci.2018.04.001
文献子类	Article
英文摘要	Mapping and isolation of quantitative trait loci (QTLs) or genes controlling grain size or weight is very important to uncover the molecular mechanisms of seed development and crop breeding. To identify the QTLs controlling grain size and weight, we developed a near isogenic line F-2 (NIL-F-2) population, which was derived from a residual heterozygous plant in an F-7 generation of recombinant inbred line (RIL). With the completion of more than 30x whole genome re-sequencing of the parents, two DNA bulks for large and small grains, a total of 58.94 Gb clean nucleotide data were generated. A total of 455 262 single nucleotide polymorphisms (SNPs) between the parents were identified to perform bulked QTL-seq. A candidate genomic region containing SNPs strongly associated with grain length and weight was identified from 15 to 20 Mb on chromosome 5. We designated the major QTL in the candidate region as qTGW5.3. Then, qTGW5.3 was further validated with PCR-based conventional QTL mapping method through developing simple sequence repeat and Insertion/Deletion markers in the F-2 population. Furthermore, recombinants and the progeny tests delimited the candidate region of qTGW5.3 to 1.13 Mb, flanked by HX5009 (15.15 Mb) and HX5003 (16.28 Mb). A set of NILs, selected from the F-2 population, was developed to evaluate the genetic effect of qTGW5.3. Significant QTL effects were detected on grain length, grain width and 1000-grain weight of H12-29 allele with 1.14 mm, -0.11 mm and 3.11 g, which explained 99.64%, 95.51% and 97.32% of the phenotypic variations, respectively.
学科主题	Agronomy ; Plant Sciences
电子版国际标准刊号	1876-4762
出版地	AMSTERDAM
WOS关键词	QUANTITATIVE TRAIT LOCI ; SIZE DIVERSITY ; MARKERS ; YIELD ; CONTRIBUTES ; DISSECTION ; PROGRESS ; ALLELE ; POOLS ; SHAPE
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000432465300001
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371605] ; Chinese High-Yielding Transgenic Program [2016ZX08001-004]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20700]
专题	中科院植物分子生理学重点实验室
作者单位	1.Fujian Acad Agr Sci, Fuzhou Natl Subctr Rice Improvement, Inst Rice Res, Fuzhou 350018, Fujian, Peoples R China 2.China Natl Rice Res Inst, Chinese Natl Ctr Rice Improvement, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Qin Yaobin,Cheng Peng,Cheng Yichen,et al. QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F-2 Population[J]. RICE SCIENCE,2018,25(3):121-131.
APA	Qin Yaobin.,Cheng Peng.,Cheng Yichen.,Feng Yue.,Huang Derun.,...&Ying Jiezheng.(2018).QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F-2 Population.RICE SCIENCE,25(3),121-131.
MLA	Qin Yaobin,et al."QTL-Seq Identified a Major QTL for Grain Length and Weight in Rice Using Near Isogenic F-2 Population".RICE SCIENCE 25.3(2018):121-131.