Nucleus-Encoded Protein BFA1 Promotes Efficient Assembly of the Chloroplast ATP Synthase Coupling Factor 1

doi:10.1105/tpc.18.00075

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	Nucleus-Encoded Protein BFA1 Promotes Efficient Assembly of the Chloroplast ATP Synthase Coupling Factor 1
	Zhang, Lin ; Pu, Hua 5; Duan, Zhikun ; Li, Yonghong 5; Liu, Bei 5; Zhang, Qiqi ; Li, Wenjing 5; Rochaix, Jean-David ; Liu, Lin 2; Peng, Lianwei
刊名	PLANT CELL
	2018
卷号	30 期号:8 页码:1770-1788
ISSN号	1040-4651
DOI	10.1105/tpc.18.00075
文献子类	Article
英文摘要	F-type ATP synthases produce nearly all of the ATP found in cells. The catalytic module F-1 commonly comprises an a alpha(3)beta(3) hexamer surrounding a gamma/epsilon stalk. However, it is unclear how these subunits assemble to form a catalytic motor. In this work, we identified and characterized a chloroplast protein that interacts with the CF1 beta, gamma, and epsilon subunits of the chloroplast ATP synthase and is required for assembly of its F-1 module. We named this protein BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE1 (BFA1) and determined its crystal structure at 2.8-angstrom resolution. BFA1 is comprised primarily of two interacting beta-barrels that are oriented nearly perpendicularly to each other. The contact region between BFA1 and the CF1 beta and gamma subunits was further mapped by yeast two-hybrid assays. An in silico molecular docking analysis was performed and revealed close fitting contact sites without steric conflicts between BFA1 and CF1 beta/gamma. We propose that BFA1 acts mainly as a scaffold protein promoting the association of a CF1 alpha/beta heterodimer with CF1 gamma. The subsequent assembly of other CF1 alpha/beta heterodimers may shift the position of the CF1 gamma. subunit to complete assembly of the CF1 module. This CF1 assembly process is likely to be valid for other F-type ATP synthases, as their structures are highly conserved.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
电子版国际标准刊号	1532-298X
出版地	ROCKVILLE
WOS关键词	ELECTRON-TRANSPORT ; EPSILON-SUBUNIT ; IN-VITRO ; ARABIDOPSIS ; BIOGENESIS ; COMPLEX ; BINDING ; PHOTOPROTECTION ; TRANSFORMATION ; ASSIMILATION
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000444293200013
资助机构	National Natural Science Foundation of China Funds for Outstanding Youth [31322007] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31700202] ; Shanghai Engineering Research Center of Plant Germplasm Resources [17DZ2252700] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2017M621511] ; Shanghai Normal University [SK201636]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20646]
专题	中科院光生物学重点实验室
作者单位	1.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China 2.Univ Geneva, Dept Plant Biol, CH-1211 Geneva, Switzerland 3.Univ Geneva, Dept Mol Biol, CH-1211 Geneva, Switzerland 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 5.Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lin,Pu, Hua,Duan, Zhikun,et al. Nucleus-Encoded Protein BFA1 Promotes Efficient Assembly of the Chloroplast ATP Synthase Coupling Factor 1[J]. PLANT CELL,2018,30(8):1770-1788.
APA	Zhang, Lin.,Pu, Hua.,Duan, Zhikun.,Li, Yonghong.,Liu, Bei.,...&Peng, Lianwei.(2018).Nucleus-Encoded Protein BFA1 Promotes Efficient Assembly of the Chloroplast ATP Synthase Coupling Factor 1.PLANT CELL,30(8),1770-1788.
MLA	Zhang, Lin,et al."Nucleus-Encoded Protein BFA1 Promotes Efficient Assembly of the Chloroplast ATP Synthase Coupling Factor 1".PLANT CELL 30.8(2018):1770-1788.