CsMYB5a and CsMYB5e from Camellia sinensis differentially regulate anthocyanin and proanthocyanidin biosynthesis

doi:10.1016/j.plantsci.2018.02.009

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	CsMYB5a and CsMYB5e from Camellia sinensis differentially regulate anthocyanin and proanthocyanidin biosynthesis
	Jiang, Xiaolan ; Huang, Keyi 4; Zheng, Guangshun 1,2,3; Hou, Hua 4; Wang, Peiqiang ; Jiang, Han ; Zhao, Xuecheng 4; Li, Mingzhuo ; Zhang, Shuxiang 4; Liu, Yajun 4
刊名	PLANT SCIENCE
	2018
卷号	270 页码:209-220
关键词	Camellia sinensis Proanthocyanidin Anthocyanin CsMYB5a and CsMYB5e Polygenic regulatory mechanism
ISSN号	0168-9452
DOI	10.1016/j.plantsci.2018.02.009
文献子类	Article
英文摘要	Tea is one of the most widely consumed nonalcoholic beverages worldwide. Polyphenols are nutritional compounds present in the leaves of tea plants. Although numerous genes are functionally characterized to encode enzymes that catalyze the formation of diverse polyphenolic metabolites, transcriptional regulation of those different pathways such as late steps of the proanthcoyanidin (PA) pathway remains unclear. In this study, using different tea transcriptome databases, we screened at least 140 R2R3-MYB transcription factors (TFs) and grouped them according to the basic function domains of the R2R3 MYB TF superfamily. Among 140 R2R3 TFs, CsMYB5a and CsMYB5e were chosen for analysis because they may be involved in PA biosynthesis regulation. CsMYB5a-overexpressing tobacco plants exhibited downregulated anthocyanin accumulation but a high polymeric PA content in the flowers. Overexpression of CsMYB5e in tobacco plants did not change the anthocyanin content but increased the dimethylaminocinnamaldehyde-stained PA content. RNA-seq and qRT-PCR analyses revealed that genes related to PA and anthocyanin biosynthesis pathways were markedly upregulated in both CsMYB5a- and CsMYB5e-overexpressing flowers. Three UGTs and four GSTs were identified as involved in PA and anthocyanin glycosylation and transportation in transgenic plants. These results provide new insights into the regulation of PA and anthocyanin biosynthesis in Camellia sinensis.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1873-2259
出版地	CLARE
WOS关键词	MYB TRANSCRIPTION FACTOR ; SEED COAT ; FUNCTIONAL-CHARACTERIZATION ; LOW-TEMPERATURE ; GENE ENCODES ; EXPRESSION ; GRAPEVINE ; REDUCTASE ; TT2 ; ACCUMULATION
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000430777600021
资助机构	Natural Science Foundation of Anhui ProvinceNatural Science Foundation of Anhui Province [1708085QC51] ; Collegiate Natural Science Foundation of Anhui Province [KJ2016A223] ; Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470689, 31570694, 31300577] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [BS2015NY003] ; Biology Key Subject Construction of Anhui
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20628]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Anhui Agr Univ, Sch Life Sci, Hefei, Anhui, Peoples R China 4.Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, Hefei, Anhui, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Qingdao Agr Univ, Coll Hort, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Xiaolan,Huang, Keyi,Zheng, Guangshun,et al. CsMYB5a and CsMYB5e from Camellia sinensis differentially regulate anthocyanin and proanthocyanidin biosynthesis[J]. PLANT SCIENCE,2018,270:209-220.
APA	Jiang, Xiaolan.,Huang, Keyi.,Zheng, Guangshun.,Hou, Hua.,Wang, Peiqiang.,...&Xia, Tao.(2018).CsMYB5a and CsMYB5e from Camellia sinensis differentially regulate anthocyanin and proanthocyanidin biosynthesis.PLANT SCIENCE,270,209-220.
MLA	Jiang, Xiaolan,et al."CsMYB5a and CsMYB5e from Camellia sinensis differentially regulate anthocyanin and proanthocyanidin biosynthesis".PLANT SCIENCE 270(2018):209-220.