ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings

doi:10.1093/jxb/ery139

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	ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings
	Jiang, Tian 2; Zhang, Jing 2; Rong, Liwei 2; Feng, Yanjiang 1; Wang, Qi 1; Song, Qiulai 1; Zhang, Lixin 2; Ouyang, Min
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2018
卷号	69 期号:12 页码:3037-3051
关键词	Arabidopsis chloroplast development cotyledon Early Chloroplast Development 1 (ECD1) early stages pentatricopeptide repeat protein (PPR) RNA editing
ISSN号	0022-0957
DOI	10.1093/jxb/ery139
文献子类	Article
英文摘要	Chloroplast development is a highly complex process and the regulatory mechanisms have not yet been fully characterized. In this study, we identified Early Chloroplast Development 1 (ECD1), a chloroplast-localized pentatricopeptide repeat protein (PPR) belonging to the PLS subfamily. Inactivation of ECD1 in Arabidopsis led to embryo lethality, and abnormal embryogenesis occurred in ecdl/+ heterozygous plants. A decrease in ECD1 expression induced by RNAi resulted in seedlings with albino cotyledons but normal true leaves. The aberrant morphology and under-developed thylakoid membrane system in cotyledons of RNAi seedlings suggests a role of ECD1 specifically in chloroplast development in seedlings. In cotyledons of ECD1-RNAi plants, RNA-editing of rps14-149 (encoding ribosomal protein S14) was seriously impaired. In addition, dramatically decreased plastid-encoded RNA polymerase-dependent gene expression and abnormal chloroplast rRNA processing were also observed. Taken together, our results indicate that ECD1 is indispensable for chloroplast development at the seedling stage in Arabidopsis.
学科主题	Plant Sciences
电子版国际标准刊号	1460-2431
出版地	OXFORD
WOS关键词	PENTATRICOPEPTIDE REPEAT PROTEINS ; RIBOSOME-DEFICIENT PLASTIDS ; GENE-EXPRESSION ; ARABIDOPSIS-THALIANA ; PPR PROTEIN ; DIFFERENTIAL REGULATION ; EMBRYO DEVELOPMENT ; PLANT DEVELOPMENT ; ESCHERICHIA-COLI ; MESSENGER-RNA
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000434047800015
资助机构	State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150100] ; Youth Innovation Promotion Association CAS [2013059]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20573]
专题	中科院光生物学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 3.Heilongjiang Acad Agr Sci, Cultivat & Crop Tillage Inst, Harbin 150086, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Tian,Zhang, Jing,Rong, Liwei,et al. ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(12):3037-3051.
APA	Jiang, Tian.,Zhang, Jing.,Rong, Liwei.,Feng, Yanjiang.,Wang, Qi.,...&Ouyang, Min.(2018).ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings.JOURNAL OF EXPERIMENTAL BOTANY,69(12),3037-3051.
MLA	Jiang, Tian,et al."ECD1 functions as an RNA-editing trans-factor of rps14-149 in plastids and is required for early chloroplast development in seedlings".JOURNAL OF EXPERIMENTAL BOTANY 69.12(2018):3037-3051.