Genome-Wide Identification of Arabidopsis LBD29 Target Genes Reveals the Molecular Events behind Auxin-Induced Cell Reprogramming during Callus Formation

doi:10.1093/pcp/pcx168

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	Genome-Wide Identification of Arabidopsis LBD29 Target Genes Reveals the Molecular Events behind Auxin-Induced Cell Reprogramming during Callus Formation
	Xu, Chongyi ; Cao, Huifen 1; Xu, Enjun ; Zhang, Shiqi 1; Hu, Yuxin 2
刊名	PLANT AND CELL PHYSIOLOGY
	2018
卷号	59 期号:4 页码:749-760
关键词	Arabidopsis thaliana Auxin Callus formation Cell reprogramming LBD
ISSN号	0032-0781
DOI	10.1093/pcp/pcx168
文献子类	Article
英文摘要	Auxin-induced callus formation represents an important cell reprogramming process during in vitro regeneration of plants, in which the pericycle or pericycle-like cells within plant organs are reprogrammed into the pluripotent cell mass termed callus that is generally required for subsequent regeneration of root or shoot. However, the molecular events behind cell reprogramming during auxin-induced callus formation are largely elusive. We previously identified that auxin-induced LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factors act as the master regulators to trigger auxin-induced callus formation. Here, by ChIP-seq (chromatin immunoprecipitation-based sequencing) and RNA sequencing approaches, we identified the potential LBD29 target genes at the genome-wide level and outlined the molecular events of LBD-triggered cell reprogramming during callus formation. We showed that LBD29 preferentially bound to the G-box (CACGTG) and TGGGC[C/T] motifs and potentially targeted > 350 genes, among which the genes related to methylation, reactive oxygen species (ROS) metabolism, cell wall hydrolysis and lipid metabolism were rapidly activated, while most of the light-responsive genes were suppressed by LBD29. Further examination of a few representative genes validated that they were targeted by LBD29 and participated in the regulation of cell reprogramming during callus formation. Our data not only outline a framework of the early molecular events behind auxin-induced cell reprogramming of callus formation, but also provide a valuable resource for identification of genes that regulate cell fate switch during in vitro regeneration of plants.
学科主题	Plant Sciences ; Cell Biology
电子版国际标准刊号	1471-9053
出版地	OXFORD
WOS关键词	NOVO ROOT ORGANOGENESIS ; SHOOT DEVELOPMENT ; BOUNDARIES ; TRANSCRIPTOME ; REGENERATION ; REPRESSION ; EXPRESSION ; EMERGENCE ; PROTEINS ; OXYGEN
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000430163300010
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31230009, 31771632] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2013CB967300] ; Strategic Priority Research Program of Chinese Academy of SciencesChinese Academy of Sciences [XDPB0403]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20454]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Xu, Chongyi,Cao, Huifen,Xu, Enjun,et al. Genome-Wide Identification of Arabidopsis LBD29 Target Genes Reveals the Molecular Events behind Auxin-Induced Cell Reprogramming during Callus Formation[J]. PLANT AND CELL PHYSIOLOGY,2018,59(4):749-760.
APA	Xu, Chongyi,Cao, Huifen,Xu, Enjun,Zhang, Shiqi,&Hu, Yuxin.(2018).Genome-Wide Identification of Arabidopsis LBD29 Target Genes Reveals the Molecular Events behind Auxin-Induced Cell Reprogramming during Callus Formation.PLANT AND CELL PHYSIOLOGY,59(4),749-760.
MLA	Xu, Chongyi,et al."Genome-Wide Identification of Arabidopsis LBD29 Target Genes Reveals the Molecular Events behind Auxin-Induced Cell Reprogramming during Callus Formation".PLANT AND CELL PHYSIOLOGY 59.4(2018):749-760.