Trichoderma asperellum ACCC30536 inoculation improves soil nutrition and leaf artemisinin production in Artemisia annua

doi:10.1007/s11738-019-2836-7

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	Trichoderma asperellum ACCC30536 inoculation improves soil nutrition and leaf artemisinin production in Artemisia annua
	Zhai, Tongtong ; Wang, Yuefeng 3; Liu, Changli 2; Liu, Zhaoying ; Zhao, Min 2; Chang, Yuan ; Zhang, Rongshu
刊名	ACTA PHYSIOLOGIAE PLANTARUM
	2019
卷号	41 期号:4
关键词	Trichoderma asperellum Artemisia annua Artemisinin Soil fertility Leaf yield
ISSN号	0137-5881
DOI	10.1007/s11738-019-2836-7
文献子类	Article
英文摘要	Artemisia annua L. is the main source of artemisinin, currently the most effective treatment for malaria. However, an affordable and abundant supply of artemisinin remains elusive. Trichoderma is a biocontrol agent that stimulates plant growth and defense responses, and improves soil quality. To date, relatively few studies have focused on improving leaf biomass and artemisinin production in A. annua using Trichoderma. To investigate the role of T. asperellum ACCC30536 in improving the artemisinin yield of A. annua, field-grown A. annua was inoculated with T. asperellum conidia and grown for 60days. The results showed that leaf artemisinin concentration and dry weight were increased significantly after inoculation. The optimal inoculation dose was 200mL of conidia suspension at 1x10(7) colony-forming units (cfu)/mL, the highest artemisinin concentration was 8.83mg/g, and the highest artemisinin production was 70.6g on day 50. The results of qRT-PCR revealed that expression of genes encoding key enzymes for artemisinin biosynthesis, namely HMGR1, FPS, ADS, CYP71AV1, CPR, DBR, DXS1, and DXR1, was generally upregulated during days 20-50 following induction by Trichoderma. In addition, the moisture, pH stability, organic matter content, and availability of nitrogen, phosphorus, and kalium in inoculated soil were significantly improved. Thus, application of T. asperellum ACCC30536 may offer a novel approach for improving artemisinin production by upregulating the expression of key enzymes for artemisinin biosynthesis, increasing leaf yield, and improving soil fertility.
学科主题	Plant Sciences
电子版国际标准刊号	1861-1664
出版地	HEIDELBERG
WOS关键词	AMORPHA-4,11-DIENE SYNTHASE ; PHYTOPHTHORA-MEGAKARYA ; GENE-EXPRESSION ; BIOSYNTHESIS ; CLONING ; AVAILABILITY ; PYRAMIDALIS ; BIOCONTROL ; GROWTH ; ENZYME
WOS研究方向	Plant Sciences
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000461290100001
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [NSFC: 31370642] ; State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) [201202] ; Natural Science Foundation of Heilongjiang Province of ChinaNatural Science Foundation of Heilongjiang Province [C201216]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19852]
专题	中科院光生物学重点实验室
作者单位	1.Northeast Forestry Univ, Coll Life Sci, 26 Hexing Rd, Harbin 150040, Heilongjiang, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 3.Northeast Forestry Univ, Coll Landscape Architecture, Harbin 150040, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Zhai, Tongtong,Wang, Yuefeng,Liu, Changli,et al. Trichoderma asperellum ACCC30536 inoculation improves soil nutrition and leaf artemisinin production in Artemisia annua[J]. ACTA PHYSIOLOGIAE PLANTARUM,2019,41(4).
APA	Zhai, Tongtong.,Wang, Yuefeng.,Liu, Changli.,Liu, Zhaoying.,Zhao, Min.,...&Zhang, Rongshu.(2019).Trichoderma asperellum ACCC30536 inoculation improves soil nutrition and leaf artemisinin production in Artemisia annua.ACTA PHYSIOLOGIAE PLANTARUM,41(4).
MLA	Zhai, Tongtong,et al."Trichoderma asperellum ACCC30536 inoculation improves soil nutrition and leaf artemisinin production in Artemisia annua".ACTA PHYSIOLOGIAE PLANTARUM 41.4(2019).