A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division

doi:10.1073/pnas.1819345116

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	A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division
	Yang, Kezhen ; Zhu, Lingling 6; Wang, Hongzhe ; Jiang, Min ; Xiao, Chunwang 7,8; Hu, Xiangyang 9; Vanneste, Steffen 1,2,3; Dong, Juan 5; Le, Jie 6
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2019
卷号	116 期号:36 页码:18126-18131
关键词	stomatal development cell division replication protein A CDK DNA damage
ISSN号	0027-8424
DOI	10.1073/pnas.1819345116
文献子类	Article
英文摘要	The R2R3-MYB transcription factor FOUR LIPS (FLP) controls the stomatal terminal division through transcriptional repression of the cell cycle genes CYCLIN-DEPENDENT KINASE (CDK) B1s (CDKB1s), CDKA; 1, and CYCLIN A2s (CYCA2s). We mutagenized the weak mutant allele flp-1 seeds with ethylmethane sulfonate and screened out a flp-1 suppressor 1 (fsp1) that suppressed the flp-1 stomatal cluster phenotype. FSP1 encodes RPA2a subunit of Replication Protein A (RPA) complexes that play important roles in DNA replication, recombination, and repair. Here, we show that FSP1/RPA2a functions together with CDKB1s and CYCA2s in restricting stomatal precursor proliferation, ensuring the stomatal terminal division and maintaining a normal guard-cell size and DNA content. Furthermore, we provide direct evidence for the existence of an evolutionarily conserved, but plant-specific, CDK-mediated RPA regulatory pathway. Serine-11 and Serine-21 at the N terminus of RPA2a are CDK phosphorylation target residues. The expression of the phosphorylation-mimic variant RPA2a(S11,21/D) partially complemented the defective cell division and DNA damage hypersensitivity in cdkb1;1 1;2 mutants. Thus, our study provides a mechanistic understanding of the CDK-mediated phosphorylation of RPA in the precise control of cell cycle and DNA repair in plants.
学科主题	Multidisciplinary Sciences
出版地	WASHINGTON
WOS关键词	CYCLIN-DEPENDENT KINASE ; RPA PHOSPHORYLATION ; CELL-PROLIFERATION ; DAMAGE RESPONSE ; 4 LIPS ; SUBUNIT ; BINDING ; REPAIR ; CDC2 ; MUTATIONS
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000485140300076
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31071198, 30971652, 31271463, 31470348] ; Research Foundation FlandersFWO ; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01GM109080] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM109080] Funding Source: NIH RePORTER
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19841]
专题	中科院植物分子生理学重点实验室
作者单位	1.Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai 200444, Peoples R China 2.VIB, Ctr Plant Syst Biol, B-9052 Ghent, Belgium 3.Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium 4.Rutgers State Univ, Waksman Inst Microbiol, Piscataway, NJ 08854 USA 5.Ghent Univ Global Campus, Lab Plant Growth Anal, Incheon 21985, South Korea 6.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 8.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China 9.Minzu Univ China, Hulun Lake Reserve Grassland Ecol Res Stn, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Yang, Kezhen,Zhu, Lingling,Wang, Hongzhe,et al. A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(36):18126-18131.
APA	Yang, Kezhen.,Zhu, Lingling.,Wang, Hongzhe.,Jiang, Min.,Xiao, Chunwang.,...&Le, Jie.(2019).A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(36),18126-18131.
MLA	Yang, Kezhen,et al."A conserved but plant-specific CDK-mediated regulation of DNA replication protein A2 in the precise control of stomatal terminal division".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.36(2019):18126-18131.