A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage

doi:10.1016/j.molp.2019.01.021

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage
	Peng, Xianjun ; Liu, Hui ; Chen, Peilin ; Tang, Feng ; Hu, Yanmin ; Wang, Fenfen ; Pi, Zhi ; Zhao, Meiling ; Chen, Naizhi ; Chen, Hui
刊名	MOLECULAR PLANT
	2019
卷号	12 期号:5 页码:661-677
关键词	forage plant barkcloth making nitrogen fixation comparative genomics genomics plant-microbe interactions
ISSN号	1674-2052
DOI	10.1016/j.molp.2019.01.021
文献子类	Article
英文摘要	Paper mulberry (Broussonetia papyrifera) is a well-known woody tree historically used for Cai Lun papermaking, one of the four great inventions of ancient China. More recently, Paper mulberry has also been used as forage to address the shortage of feedstuff because of its digestible crude fiber and high protein contents. In this study, we obtained a chromosome-scale genome assembly for Paper mulberry using integrated approaches, including Illumine and PacBio sequencing platform as well as Hi-C, optical, and genetic maps. The assembled Paper mulberry genome consists of 386.83 Mb, which is close to the estimated size, and 99.25% (383.93 Mb) of the assembly was assigned to 13 pseudochromosomes. Comparative genomic analysis revealed the expansion and contraction in the flavonoid and lignin biosynthetic gene families, respectively, accounting for the enhanced flavonoid and decreased lignin biosynthesis in Paper mulberry. Moreover, the increased ratio of syringyl-lignin to guaiacyl-lignin in Paper mulberry underscores its suitability for use in medicine, forage, papermaking, and barkcloth making. We also identified the root-associated microbiota of Paper mulberry and found that Pseudomonas and Rhizobia were enriched in its roots and may provide the source of nitrogen for its stems and leaves via symbiotic nitrogen fixation. Collectively, these results suggest that Paper mulberry might have undergone adaptive evolution and recruited nitrogen-fixing microbes to promote growth by enhancing flavonoid production and altering lignin monomer composition. Our study provides significant insights into genetic basis of the usefulness of Paper mulberry in papermaking and barkcloth making, and as forage. These insights will facilitate further domestication and selection as well as industrial utilization of Paper mulberry worldwide.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1752-9867
出版地	CAMBRIDGE
WOS关键词	DRAFT GENOME ; COMPREHENSIVE ANALYSIS ; PHYLOGENETIC ANALYSIS ; SEQUENCE ; LIGNIN ; IDENTIFICATION ; PROTEIN ; BIOSYNTHESIS ; RHIZOSPHERE ; EVOLUTION
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000466958300008
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770360, 31870247] ; Poverty Relief Project of the Chinese Academy of Sciences [KFJ-FP-24] ; Huimin Technology Demonstration Project of the National Modern Agricultural Science and Technology Achievements City [Z151100001015008]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19649]
专题	中科院北方资源植物重点实验室
作者单位	1.Beijing Jonathan Sci & Technol Dev Co Ltd, Beijing 101314, Peoples R China 2.ChuangGou Sci & Technol Co Ltd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 4.Quick Green Biotec Co Ltd, Dalian 116600, Peoples R China 5.Biomarker Technol Corp, Beijing 101300, Peoples R China
推荐引用方式 GB/T 7714	Peng, Xianjun,Liu, Hui,Chen, Peilin,et al. A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage[J]. MOLECULAR PLANT,2019,12(5):661-677.
APA	Peng, Xianjun.,Liu, Hui.,Chen, Peilin.,Tang, Feng.,Hu, Yanmin.,...&Shen, Shihua.(2019).A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage.MOLECULAR PLANT,12(5),661-677.
MLA	Peng, Xianjun,et al."A Chromosome-Scale Genome Assembly of Paper Mulberry (Broussonetia papyrifera) Provides New Insights into Its Forage and Papermaking Usage".MOLECULAR PLANT 12.5(2019):661-677.