Comparative Study Reveals Insights of Sheepgrass (Leymus chinensis) Coping With Phosphate-Deprived Stress Condition

doi:10.3389/fpls.2019.00170

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	Comparative Study Reveals Insights of Sheepgrass (Leymus chinensis) Coping With Phosphate-Deprived Stress Condition
	Li, Lingyu 4; Yang, Haomeng ; Peng, Lianwei 5; Ren, Weibo 1; Gong, Jirui 2; Liu, Peng 4; Wu, Xinhong 1; Huang, Fang
刊名	FRONTIERS IN PLANT SCIENCE
	2019
卷号	10
关键词	L. chinensis Pi-deprivation low-Pi stress ATP synthase photosynthesis
ISSN号	1664-462X
DOI	10.3389/fpls.2019.00170
文献子类	Article
英文摘要	Sheepgrass [Leymus chinensis (Trin.) Tzvel] is a valuable forage plant highly significant to the grassland productivity of Euro-Asia steppes. Growth of above-ground tissues of L. chinensis is the major component contributing to the grass yield. Although it is generally known that this species is sensitive to ecosystem disturbance and adverse environments, detailed information of how L. chinensis coping with various nutrient deficiency especially phosphate deprivation (-Pi) is still limited. Here, we investigated impact of Pi-deprivation on shoot growth and biomass accumulation as well as photosynthetic properties of L. chinensis. Growth inhibition of Pi-deprived seedlings was most obvious and reduction of biomass accumulation and net photosynthetic rate (Pn) was 55.3 and 63.3%, respectively, compared to the control plants grown under Pi-repleted condition. Also, we compared these characters with seedlings subjected to low-Pi stress condition. Pi-deprivation caused 18.5 and 12.3% more reduction of biomass and Pn relative to low-Pi-stressed seedlings, respectively. Further analysis of in vivo chlorophyll fluorescence and thylakoid membrane protein complexes using 2D-BN/SDS-PAGE combined with immunoblot detection demonstrated that among the measured photosynthetic parameters, decrease of ATP synthase activity was most pronounced in Pi-deprived plants. Together with less extent of lipid peroxidation of the thylakoid membranes and increased ROS scavenger enzyme activities in the leaves of Pi-deprived seedlings, we suggest that the decreased activity of ATP synthase in their thylakoids is the major cause of the greater reduction of photosynthetic efficiency than that of low-Pi stressed plants, leading to the least shoot growth and biomass production in L. chinensis.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	LIPID-PEROXIDATION ; PHOTOSYSTEM-I ; ARABIDOPSIS ; EXPRESSION ; STARVATION ; RESPONSES ; PROTEIN ; ROOTS ; ASSAY ; PHOTOSYNTHESIS
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000458949200001
资助机构	National Key Basic Research Development Program of ChinaNational Basic Research Program of China [2014CB138800] ; Chinese Academy of SciencesChinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19604]
专题	中科院光生物学重点实验室
作者单位	1.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai, Peoples R China 2.Chinese Acad Agr Sci, Inst Grassland Res, Hohhot, Peoples R China 3.Beijing Normal Univ, Coll Resources Sci & Technol, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Lingyu,Yang, Haomeng,Peng, Lianwei,et al. Comparative Study Reveals Insights of Sheepgrass (Leymus chinensis) Coping With Phosphate-Deprived Stress Condition[J]. FRONTIERS IN PLANT SCIENCE,2019,10.
APA	Li, Lingyu.,Yang, Haomeng.,Peng, Lianwei.,Ren, Weibo.,Gong, Jirui.,...&Huang, Fang.(2019).Comparative Study Reveals Insights of Sheepgrass (Leymus chinensis) Coping With Phosphate-Deprived Stress Condition.FRONTIERS IN PLANT SCIENCE,10.
MLA	Li, Lingyu,et al."Comparative Study Reveals Insights of Sheepgrass (Leymus chinensis) Coping With Phosphate-Deprived Stress Condition".FRONTIERS IN PLANT SCIENCE 10(2019).