Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis

doi:10.1111/tpj.14302

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis
	Liu, Jin ; Sun, Zheng 2; Mao, Xuemei ; Gerken, Henri 1; Wang, Xiaofei ; Yang, Wenqiang 4
刊名	PLANT JOURNAL
	2019
卷号	98 期号:6 页码:1060-1077
关键词	Chromochloris zofingiensis glycerol-3-phosphate acyltransferase oleaginousness multiomics trait improvement triacylglycerol
ISSN号	0960-7412
DOI	10.1111/tpj.14302
文献子类	Article
英文摘要	Chromochloris zofingiensis, featured due to its capability to simultaneously synthesize triacylglycerol (TAG) and astaxanthin, is emerging as a leading candidate alga for production uses. To better understand the oleaginous mechanism of this alga, we conducted a multiomics analysis by systematically integrating time-resolved transcriptomes, lipidomes and metabolomes in response to nitrogen deprivation. The data analysis unraveled the distinct mechanism of TAG accumulation, which involved coordinated stimulation of multiple biological processes including supply of energy and reductants, carbon reallocation from protein and starch, and 'pushing' and 'pulling' carbon to TAG synthesis. Unlike the model alga Chlamydomonas, de novo fatty acid synthesis in C. zofingiensis was promoted, together with enhanced turnover of both glycolipids and phospholipids, supporting the drastic need of acyls for TAG assembly. Moreover, genomewide analysis identified many key functional enzymes and transcription factors that had engineering potential for TAG modulation. Two genes encoding glycerol-3-phosphate acyltransferase (GPAT), the first committed enzyme for TAG assembly, were found in the C. zofingiensis genome; in vivo functional characterization revealed that extrachloroplastic GPAT instead of chloroplastic GPAT played a central role in TAG synthesis. These findings illuminate distinct oleaginousness mechanisms in C. zofingiensis and pave the way towards rational manipulation of this alga to becone an emerging model for trait improvements.
学科主题	Plant Sciences
电子版国际标准刊号	1365-313X
出版地	HOBOKEN
WOS关键词	MICROALGA CHLORELLA-ZOFINGIENSIS ; PENTOSE-PHOSPHATE PATHWAY ; CHLAMYDOMONAS-REINHARDTII ; DIACYLGLYCEROL ACYLTRANSFERASES ; NITROGEN DEPRIVATION ; LIPID-ACCUMULATION ; ACID ACYLTRANSFERASE ; PHYTOENE DESATURASE ; BIOFUEL PRODUCTION ; OIL SYNTHESIS
WOS研究方向	Plant Sciences
语种	英语
出版者	WILEY
WOS记录号	WOS:000473644100009
资助机构	National Youth Thousand Talents Program ; Peking University CCUS project - BHP Billiton ; Double-First Class Initiative of Peking University
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/19544]
专题	中科院光生物学重点实验室
作者单位	1.Shanghai Ocean Univ, Minist Sci & Technol, Int Res Ctr Marine Biosci, Shanghai 201306, Peoples R China 2.Peking Univ, Coll Engn, Lab Algae Biotechnol & Innovat, Beijing 100871, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynthesis Res Ctr, Beijing 100093, Peoples R China 4.Arizona State Univ Polytech Campus, Sch Sustainable Engn & Built Environm, Mesa, AZ 85212 USA
推荐引用方式 GB/T 7714	Liu, Jin,Sun, Zheng,Mao, Xuemei,et al. Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis[J]. PLANT JOURNAL,2019,98(6):1060-1077.
APA	Liu, Jin,Sun, Zheng,Mao, Xuemei,Gerken, Henri,Wang, Xiaofei,&Yang, Wenqiang.(2019).Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis.PLANT JOURNAL,98(6),1060-1077.
MLA	Liu, Jin,et al."Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis".PLANT JOURNAL 98.6(2019):1060-1077.