Anti-inflammatory effect of Acalypha australis L. via suppression of NF-kappa B signaling in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice

doi:10.1016/j.molimm.2020.01.010

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	Anti-inflammatory effect of Acalypha australis L. via suppression of NF-kappa B signaling in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice
	Kim, Hyo-Jung ; Joe, Hae-In ; Zhang, Zhiyun 2; Lee, Sang Woo 3; Lee, Kyou-Young ; Kook, Yoon-Bum ; An, Hyo-Jin
刊名	MOLECULAR IMMUNOLOGY
	2020
卷号	119 页码:123-131
关键词	Acalypha australis L. Anti-inflammation Lipopolysaccharide NF-kappa B RAW 264.7 macrophages Septic shock
ISSN号	0161-5890
DOI	10.1016/j.molimm.2020.01.010
文献子类	Article
英文摘要	We evaluated the anti-inflammatory activity of methanol extracts of Chinese medicinal plants from Beijing and determined which extract was the most effective. We found the methanol extract of Acalypha auswalis L. (AAL) to be the most effective. AAL has been used for clearing heat, toxic material, and hemostasia in Chinese medicine. Although these uses are closely related to inflammation, the anti-inflammatory effect of AAL has not yet been described and its underlying mechanism remains unclear. Therefore, we aimed to identify anti-inflammatory effect of AAL and its underlying mechanism in vitro and in vivo. In RAW 264.7 macrophages, cytotoxicity was evaluated by MTT assay and nitric oxide (NO) was measured with Griess reagent. To confirm the production of pro-inflammatory cytokines and its mRNA expression, enzyme immunoassay (ETA) and quantitative real-time PCR (qRT-PCR) were performed. Further, protein expression was analyzed by western blotting. Septic shock was induced by intraperitoneal injection of LPS (25 mg/kg) in mice. One hour before LPS injection, AAL (25 and 50 mg/kg) was administered orally. In LPS-stimulated macrophages, AAL inhibited NO production at concentrations without cytotoxicity. Additionally, AAL reduced not only inducible nitric oxide synthase (iNOS) expression but the production of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) by attenuating nuclear factor-kappa B (NF-kappa B)-related proteins (NF-kappa B p65, phosphorylation of inhibitor kappa B-alpha; p-I kappa B-alpha, phosphorylation of inhibitor kappa B kinase-alpha/beta; p-Ikk-alpha/beta). Moreover, AAL enhanced the survival rate of mice through the inhibition of iNOS expression and IL-6 and interleukin-1 beta (IL-1 beta) production in LPS-induced septic mice. Furthermore, AAL also reduced the expression of NF-kappa B-related proteins. These finding suggest that AAL is related to the modulation of inflammatory reactions by blocking NF-kappa B activation in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice.
学科主题	Biochemistry & Molecular Biology ; Immunology
出版地	OXFORD
WOS关键词	INFLAMMATORY MEDIATORS ; ENDOTOXIC-SHOCK ; NITRIC-OXIDE ; INACTIVATION ; INHIBITION ; ACTIVATION ; RESPONSES ; PROTECTS ; LINN.
WOS研究方向	Biochemistry & Molecular Biology ; Immunology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000517657900015
资助机构	National Research Foundation of Korea (NRF) - the Korea government (MSIP ; Ministry of Science, ICT & Future Planning) [NRF-2018R1C1B5041014]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21888]
专题	系统与进化植物学国家重点实验室
作者单位	1.Sangji Univ, Coll Korean Med, Dept Pharmacol, 83 Sangjidae Gil, Wonju 26339, Gangwon Do, South Korea 2.Sangji Univ, Coll Korean Med, Dept Prescript, 83 Sangjidae Gil, Wonju 26339, Gangwon Do, South Korea 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Korea Res Inst Biosci & Biotechnol, Int Biol Mat Res Ctr, Daejeon 34141, South Korea 5.Sangji Univ, Coll Korean Med, Dept Korean Ophthalmol & Otolaryngol & Dermatol, 83 Sangjidae Gil, Wonju 26339, Gangwon Do, South Korea
推荐引用方式 GB/T 7714	Kim, Hyo-Jung,Joe, Hae-In,Zhang, Zhiyun,et al. Anti-inflammatory effect of Acalypha australis L. via suppression of NF-kappa B signaling in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice[J]. MOLECULAR IMMUNOLOGY,2020,119:123-131.
APA	Kim, Hyo-Jung.,Joe, Hae-In.,Zhang, Zhiyun.,Lee, Sang Woo.,Lee, Kyou-Young.,...&An, Hyo-Jin.(2020).Anti-inflammatory effect of Acalypha australis L. via suppression of NF-kappa B signaling in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice.MOLECULAR IMMUNOLOGY,119,123-131.
MLA	Kim, Hyo-Jung,et al."Anti-inflammatory effect of Acalypha australis L. via suppression of NF-kappa B signaling in LPS-stimulated RAW 264.7 macrophages and LPS-induced septic mice".MOLECULAR IMMUNOLOGY 119(2020):123-131.