Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature

doi:10.1016/j.envexpbot.2020.104216

CORC > 植物研究所 > 中国科学院植物研究所 > 植被与环境变化国家重点实验室

	Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature
	Li, Qian ; Yang, An 2
刊名	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
	2020
卷号	179
关键词	Rice germination Low temperature Gibberellins Abscisic acid alpha-Amylase Soluble sugar
ISSN号	0098-8472
DOI	10.1016/j.envexpbot.2020.104216
文献子类	Article
英文摘要	Seed germination is a key phase of the plant life cycle, and the plant is highly sensitive to low temperature during this phase. However, the physiological and molecular mechanisms underlying the adaptation of rice seeds to low temperatures remain elusive. The present study compared seed germination between Dongdao-4 and Jigeng-88 and investigated the mechanisms of their response to low temperature conditions. Seed germination percentage of Dongdao-4 was significantly higher than that of Jigeng-88 when exposed to low temperature. Furthermore, Dongdao-4 seeds exhibited higher concentrations of bioactive gibberellins (GA(1)and GA(4)) under low temperature conditions compared to Jigeng-88 seeds, which was attributed to a higher induction of the expression of GA biosynthetic genes and a lower induction of the expression of GA inactivation genes at the transcriptional level in Dongdao-4 compared to those in Jigeng-88 seeds. We further demonstrated that GA treatment promoted seed germination under low temperature conditions, and GA biosynthetic inhibitors inhibited germination. During seed germination, soluble sugars are converted from starch by a-amylase to provide energy and fuel embryo development. The reduction in the germination percentage is accompanied by a reduction in a-amylase activity and soluble sugar concentrations under low temperature conditions. We observed higher a-amylase activity and soluble sugar levels in Dongdao-4 than in Jigeng-88 seeds under low temperature conditions. More soluble sugars were transported to embryos in Dongdao-4 seeds than in Jigeng-88 seeds under low temperature conditions, which was attributed to a higher transcriptional level of sugar transporters in Dongdao-4 than that in Jigeng-88. Abscisic acid (ABA) acts antagonistically compared to GA during seed germination. Lower endogenous concentrations of ABA in Dongdao-4 compared to those in Jigeng-88 seeds were found, because of the much higher transcriptional levels of ABA catabolism genes in Dongdao-4 than Jigeng-88 seeds when they were treated at an identical low temperature. These results suggest that the higher accumulation of endogenous bioactive GAs and soluble sugars and lower ABA concentrations in Dongdao-4 than Jigeng-88 were responsible for its increased seed germination under low temperature conditions.
学科主题	Plant Sciences ; Environmental Sciences
电子版国际标准刊号	1873-7307
出版地	OXFORD
WOS关键词	QUANTITATIVE TRAIT LOCUS ; TRANSPORTER GENE FAMILY ; ALPHA-AMYLASE ACTIVITY ; ABSCISIC-ACID ; SUCROSE TRANSPORTER ; SALT STRESS ; EXPRESSION ANALYSIS ; GREATER TOLERANCE ; NATURAL VARIATION ; SALINITY
WOS研究方向	Plant Sciences ; Environmental Sciences & Ecology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000571434200004
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31801280, 31301832] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2452018114] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2019M653770]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21581]
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 2.Northwest A&F Univ, Coll Hort, Yangling, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Li, Qian,Yang, An. Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature[J]. ENVIRONMENTAL AND EXPERIMENTAL BOTANY,2020,179.
APA	Li, Qian,&Yang, An.(2020).Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,179.
MLA	Li, Qian,et al."Comparative studies on seed germination of two rice genotypes with different tolerances to low temperature".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 179(2020).