Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells

doi:10.3389/fncel.2022.801644

	Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells
	Chen, Rui 2; Zhang, Ningxia 1; Zhou, Yubin 4; Jing, Ji 3
刊名	FRONTIERS IN CELLULAR NEUROSCIENCE
	2022-02-16
卷号	16
关键词	proximity labeling TurboID optogenetics OptoID biotinylation
DOI	10.3389/fncel.2022.801644
通讯作者	Zhou, Yubin(yubinzhou@tamu.edu) ; Jing, Ji(jingji@ibmc.ac.cn)
英文摘要	Proximity-dependent biotinylation techniques have been gaining wide applications in the systematic analysis of protein-protein interactions (PPIs) on a proteome-wide scale in living cells. The engineered biotin ligase TurboID is among the most widely adopted given its enhanced biotinylation efficiency, but it faces the background biotinylation complication that might confound proteomic data interpretation. To address this issue, we report herein a set of split TurboID variants that can be reversibly assembled by using light (designated "OptoID"), which enable optogenetic control of biotinylation based proximity labeling in living cells. OptoID could be further coupled with an engineered monomeric streptavidin that permits real-time monitoring of biotinylation with high temporal precision. These optical actuators and sensors will likely find broad applications in precise proximity proteomics and rapid detection of biotinylation in living cells.
资助项目	Welch Foundation[BE-1913-20190330] ; Cancer Hospital of the University of Chinese Academy of Sciences ; Institute of Basic Medicine and Cancer
WOS关键词	STREPTAVIDIN MONOMER ; MEMBRANE RECRUITMENT ; BIOTIN REPRESSOR ; DIMERIZATION ; PROTEINS ; BINDING ; GROWTH ; LIGHT ; DIMER ; TOOL
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000764307900001
资助机构	Welch Foundation ; Cancer Hospital of the University of Chinese Academy of Sciences ; Institute of Basic Medicine and Cancer
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/127855]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhou, Yubin; Jing, Ji
作者单位	1.Zhejiang Univ, Sir Run Run Shaw Hosp, Coll Med, Inst Clin Sci,Lab Canc Biol,Dept Med Oncol, Hangzhou, Peoples R China 2.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Oral & Maxillofacial Surg, Guangzhou, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp,Inst Basic Med & Canc, Hangzhou, Peoples R China 4.Texas A&M Univ, Coll Med, Ctr Translat Canc Res Inst Biosci & Technol, Dept Translat Med Sci, Houston, TX 77843 USA
推荐引用方式 GB/T 7714	Chen, Rui,Zhang, Ningxia,Zhou, Yubin,et al. Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells[J]. FRONTIERS IN CELLULAR NEUROSCIENCE,2022,16.
APA	Chen, Rui,Zhang, Ningxia,Zhou, Yubin,&Jing, Ji.(2022).Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells.FRONTIERS IN CELLULAR NEUROSCIENCE,16.
MLA	Chen, Rui,et al."Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells".FRONTIERS IN CELLULAR NEUROSCIENCE 16(2022).