Construction of Optimal SERS Hotspots Based on Capturing the Spike Receptor-Binding Domain (RBD) of SARS-CoV-2 for Highly Sensitive and Specific Detection by a Fish Model

doi:10.1021/acs.analchem.1c03807

	Construction of Optimal SERS Hotspots Based on Capturing the Spike Receptor-Binding Domain (RBD) of SARS-CoV-2 for Highly Sensitive and Specific Detection by a Fish Model
	Huang, Guangyao 1,2,3,4; Zhao, Hongxin 6; Li, Pan 3,4; Liu, Juanjuan 6; Chen, Siyu 2,3,4; Ge, Meihong 2,3,4; Qin, Miao 2,3,4; Zhou, Guoliang 2,3,4; Wang, Yongtao 2,3,4; Li, Shaofei 2,3,4
刊名	ANALYTICAL CHEMISTRY
	2021-12-07
卷号	93
ISSN号	0003-2700
DOI	10.1021/acs.analchem.1c03807
通讯作者	Li, Pan(lipan2011@iim.ac.cn) ; Wang, Hongzhi(wanghz@hfcas.ac.cn) ; Yang, Liangbao(lbyang@iim.ac.cn)
英文摘要	It is highly challenging to construct the best SERS hotspots for the detection of proteins by surface-enhanced Raman spectroscopy (SERS). Using its own characteristics to construct hotspots can achieve the effect of sensitivity and specificity. In this study, we built a fishing mode device to detect the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at low concentrations in different detection environments and obtained a sensitive SERS signal response. Based on the spatial resolution of proteins and their protein-specific recognition functions, SERS hotspots were constructed using aptamers and small molecules that can specifically bind to RBD and cooperate with Au nanoparticles (NPs) to detect RBD in the environment using SERS signals of beacon molecules. Therefore, two kinds of AuNPs modified with aptamers and small molecules were used in the fishing mode device, which can specifically recognize and bind RBD to form a stable hotspot to achieve high sensitivity and specificity for RBD detection. The fishing mode device can detect the presence of RBD at concentrations as low as 0.625 ng/mL and can produce a good SERS signal response within 15 min. Meanwhile, we can detect an RBD of 0.625 ng/mL in the mixed solution with various proteins, and the concentration of RBD in the complex environment of urine and blood can be as low as 1.25 ng/mL. This provides a research basis for SERS in practical applications for protein detection work.
资助项目	National Natural Science Foundation of China[21974142] ; Sci-tech Police Project of Anhui Province[201904d07020009] ; Nature Science Research Project of Anhui Province[1908085QB65] ; Anhui Provincial Key RD Programm[202104d07020002]
WOS关键词	ENHANCED RAMAN-SCATTERING ; FUNCTIONALIZED ACUPUNCTURE NEEDLE ; NANOPARTICLES ; DISCOVERY ; RELEASE ; SENSOR
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000753022600035
资助机构	National Natural Science Foundation of China ; Sci-tech Police Project of Anhui Province ; Nature Science Research Project of Anhui Province ; Anhui Provincial Key RD Programm
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/127609]
专题	中国科学院合肥物质科学研究院
通讯作者	Li, Pan; Wang, Hongzhi; Yang, Liangbao
作者单位	1.Chinese Acad Sci, Canc Hosp, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei 230031, Peoples R China 4.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei 230031, Peoples R China 5.Fudan Univ, Multiscale Res Inst Complex Syst, Shanghai 201203, Peoples R China 6.Chinese Acad Sci, High Magnet Field Sci Ctr, Hefei Inst Phys Sci, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Huang, Guangyao,Zhao, Hongxin,Li, Pan,et al. Construction of Optimal SERS Hotspots Based on Capturing the Spike Receptor-Binding Domain (RBD) of SARS-CoV-2 for Highly Sensitive and Specific Detection by a Fish Model[J]. ANALYTICAL CHEMISTRY,2021,93.
APA	Huang, Guangyao.,Zhao, Hongxin.,Li, Pan.,Liu, Juanjuan.,Chen, Siyu.,...&Yang, Liangbao.(2021).Construction of Optimal SERS Hotspots Based on Capturing the Spike Receptor-Binding Domain (RBD) of SARS-CoV-2 for Highly Sensitive and Specific Detection by a Fish Model.ANALYTICAL CHEMISTRY,93.
MLA	Huang, Guangyao,et al."Construction of Optimal SERS Hotspots Based on Capturing the Spike Receptor-Binding Domain (RBD) of SARS-CoV-2 for Highly Sensitive and Specific Detection by a Fish Model".ANALYTICAL CHEMISTRY 93(2021).