Rapid and Sensitive Detection of Water Toxicity Based on Photosynthetic Inhibition Effect

doi:10.3390/toxics9120321

	Rapid and Sensitive Detection of Water Toxicity Based on Photosynthetic Inhibition Effect
	Chen, Min 1,2,3; Yin, Gaofang 1,3; Zhao, Nanjing 1,3; Gan, Tingting 1,2,3; Feng, Chun 1,2,3; Gu, Mengyuan 1,2,3; Qi, Peilong 1,2,3; Ding, Zhichao 1,2,3
刊名	TOXICS
	2021-12-01
卷号	9
关键词	photosynthetic inhibition photosynthetic fluorescence parameter Chlorella pyrenoidosa toxic water pollutants rapid detection biotesting
DOI	10.3390/toxics9120321
通讯作者	Yin, Gaofang(gfyin@aiofm.ac.cn)
英文摘要	To achieve rapid and sensitive detection of the toxicity of pollutants in the aquatic environment, a photosynthetic inhibition method with microalgae as the test organism and photosynthetic fluorescence parameters as the test endpoint was proposed. In this study, eight environmental pollutants were selected to act on the tested organism, Chlorella pyrenoidosa, including herbicides (diuron, atrazine), fungicides (fuberidazole), organic chemical raw materials (phenanthrene, phenol, p-benzoquinone), disinfectants (trichloroacetonitrile uric acid), and disinfection by-products (trichloroacetonitrile). The results showed that, in addition to specific PSII inhibitors (diuretic and atrazine), other types of pollutants could also quickly affect the photosynthetic system. The photosynthetic fluorescence parameters (Fv/Fm, Yield, alpha, and rP) could be used to detect the effects of pollutants on the photosynthetic system. Although the decay rate of the photosynthetic fluorescence parameters corresponding to the different pollutants was different, 1 h could be used as an appropriate toxicity exposure time. Moreover, the lowest respondent concentrations of photosynthetic fluorescence parameters to diuron, atrazine, fuberidazole, phenanthrene, P-benzoquinone, phenol, trichloroacetonitrile uric acid, and trichloroacetonitrile were 2 mu g center dot L-1, 5 mu g center dot L-1, 0.05 mg center dot L-1, 2 mu g center dot L-1, 1.0 mg center dot L-1, 0.4 g center dot L-1, 0.1 mg center dot L-1, and 2.0 mg center dot L-1, respectively. Finally, diuron, atrazine, fuberidazole, and phenanthrene were selected for a comparison of their photosynthetic inhibition and growth inhibition. The results suggested that photosynthetic inhibition could overcome the time dependence of growth inhibition and shorten the toxic exposure time from more than 24 h to less than 1 h, or even a few minutes, while, the sensitivity of the toxicity test was not weakened. This study indicates that the photosynthetic inhibition method could be used for rapid detection of the toxicity of water pollutants and that algae fluorescence provides convenient access to toxicity data.
资助项目	National Natural Science Foundation of China[61875207] ; National Natural Science Foundation of China[61805254] ; Anhui Province Science and Technology Major Special Project[202003a07020007] ; Anhui Provincial Excellent Youth Science Foundation of China[1908085J23] ; Instrument Function Development Technology Innovation Project of Chinese Academy of Sciences[Y93H3g1251]
WOS关键词	RISK-ASSESSMENT ; GROWTH ; FLUORESCENCE ; RESPONSES ; PERFORMANCE ; POLLUTANTS ; ORGANISMS ; EXPOSURE ; TIME
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
语种	英语
出版者	MDPI
WOS记录号	WOS:000738448700001
资助机构	National Natural Science Foundation of China ; Anhui Province Science and Technology Major Special Project ; Anhui Provincial Excellent Youth Science Foundation of China ; Instrument Function Development Technology Innovation Project of Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/127147]
专题	中国科学院合肥物质科学研究院
通讯作者	Yin, Gaofang
作者单位	1.Key Lab Opt Monitoring Technol Environm Anhui Pro, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Inst Opt & Fine Mech, Key Lab Environm Opt & Technol, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Chen, Min,Yin, Gaofang,Zhao, Nanjing,et al. Rapid and Sensitive Detection of Water Toxicity Based on Photosynthetic Inhibition Effect[J]. TOXICS,2021,9.
APA	Chen, Min.,Yin, Gaofang.,Zhao, Nanjing.,Gan, Tingting.,Feng, Chun.,...&Ding, Zhichao.(2021).Rapid and Sensitive Detection of Water Toxicity Based on Photosynthetic Inhibition Effect.TOXICS,9.
MLA	Chen, Min,et al."Rapid and Sensitive Detection of Water Toxicity Based on Photosynthetic Inhibition Effect".TOXICS 9(2021).