Molecular Response to High Hydrostatic Pressure: Time-Series Transcriptomic Analysis of Shallow-Water Sea Cucumber Apostichopus japonicus

doi:10.3389/fgene.2020.00355

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋生物生态与进化研究组

	Molecular Response to High Hydrostatic Pressure: Time-Series Transcriptomic Analysis of Shallow-Water Sea Cucumber Apostichopus japonicus
	Chen, Jiawei 1,2; Liang, Linying 1,2; Li, Yanan 1,2; Zhang, Haibin 1
刊名	FRONTIERS IN GENETICS
	2020-04-30
卷号	11 页码:12
关键词	time series transcriptomic molecular response hydrostatic pressure sea cucumber
DOI	10.3389/fgene.2020.00355
英文摘要	Hydrostatic pressure is a key environmental factor constraining the benthic migration of shallow-water invertebrates. Although many studies have examined the physiological effects of high hydrostatic pressure on shallow-water invertebrates, the molecular response to high pressure is not fully understood. This question has received increasing attention because ocean warming is forcing the bathymetric migrations of shallow-water invertebrates. Here, we applied time-series transcriptomic analysis to high-pressure incubated and atmospheric pressure-recovered shallow-water sea cucumber (Apostichopus japonicus) to address this question. A total of 44 samples from 15 experimental groups were sequenced. Our results showed that most genes responded to pressure stress at the beginning when pressure was changed, but significant differences of gene expression appeared after 4 to 6 h. Transcription was the most sensitive biological process responding to high-pressure exposure, which was enriched among up-regulated genes after 2 h, followed by ubiquitination (4 h), endocytosis (6 h), stress response (6 h), methylation regulation (24 h), and transmembrane transportation (24 h). After high-pressure incubation, all these biological processes remained up-regulated within 4-6 h at atmospheric pressure. Overall, our results revealed the dynamic transcriptional response of A. japonicus to high-pressure exposure. Additionally, few quantitative or functional responses related to A. japonicus on transcriptional level were introduced by hydrostatic pressure changes after 1 h, and main biological responses were introduced after 4 h, suggesting that, when hydrostatic pressure is the mainly changed environmental factor, it will be better to fix sea cucumber samples for transcriptomic analysis within 1 h, but 4 h will be also acceptable.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences (CAS)[XDA22040502] ; National Key Research and Development Program of China[2016YFC0304905] ; National Key Research and Development Program of China[2018YFC0309804] ; Major scientific and technological projects of Hainan Province[ZDKJ2019011]
WOS关键词	INSIGHTS ; ANNOTATION ; TEMPERATURE ; ADAPTATIONS ; PACKAGE ; CLIMATE
WOS研究方向	Genetics & Heredity
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000536219700001
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) ; National Key Research and Development Program of China ; Major scientific and technological projects of Hainan Province
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/7657]
专题	深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者	Zhang, Haibin
作者单位	1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jiawei,Liang, Linying,Li, Yanan,et al. Molecular Response to High Hydrostatic Pressure: Time-Series Transcriptomic Analysis of Shallow-Water Sea Cucumber Apostichopus japonicus[J]. FRONTIERS IN GENETICS,2020,11:12.
APA	Chen, Jiawei,Liang, Linying,Li, Yanan,&Zhang, Haibin.(2020).Molecular Response to High Hydrostatic Pressure: Time-Series Transcriptomic Analysis of Shallow-Water Sea Cucumber Apostichopus japonicus.FRONTIERS IN GENETICS,11,12.
MLA	Chen, Jiawei,et al."Molecular Response to High Hydrostatic Pressure: Time-Series Transcriptomic Analysis of Shallow-Water Sea Cucumber Apostichopus japonicus".FRONTIERS IN GENETICS 11(2020):12.