Insights into the Synthesis, Secretion and Curing of Barnacle Cyprid Adhesive via Transcriptomic and Proteomic Analyses of the Cement Gland

doi:10.3390/md18040186

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海生物蛋白质组学及分子生物学研究组

	Insights into the Synthesis, Secretion and Curing of Barnacle Cyprid Adhesive via Transcriptomic and Proteomic Analyses of the Cement Gland
	Yan, Guoyong 2,3; Sun, Jin 4,5; Wang, Zishuai 1; Qian, Pei-Yuan 4,5; He, Lisheng 2
刊名	MARINE DRUGS
	2020-04-01
卷号	18 期号:4 页码:19
关键词	barnacle cement gland cyprid adhesive transcriptome cement protein
DOI	10.3390/md18040186
英文摘要	Barnacles represent one of the model organisms used for antifouling research, however, knowledge regarding the molecular mechanisms underlying barnacle cyprid cementation is relatively scarce. Here, RNA-seq was used to obtain the transcriptomes of the cement glands where adhesive is generated and the remaining carcasses of Megabalanus volcano cyprids. Comparative transcriptomic analysis identified 9060 differentially expressed genes, with 4383 upregulated in the cement glands. Four cement proteins, named Mvcp113k, Mvcp130k, Mvcp52k and Mvlcp1-122k, were detected in the cement glands. The salivary secretion pathway was significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the differentially expressed genes, implying that the secretion of cyprid adhesive might be analogous to that of saliva. Lysyl oxidase had a higher expression level in the cement glands and was speculated to function in the curing of cyprid adhesive. Furthermore, the KEGG enrichment analysis of the 352 proteins identified in the cement gland proteome partially confirmed the comparative transcriptomic results. These results present insights into the molecular mechanisms underlying the synthesis, secretion and curing of barnacle cyprid adhesive and provide potential molecular targets for the development of environmentally friendly antifouling compounds.
资助项目	National Key Research and Development Program of China[2016YFC0304905] ; National Natural Science Foundation of China[31460092] ; Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory[SMSEGL20Sc01]
WOS关键词	BALANUS-BALANOIDES ; MOLECULAR-MECHANISMS ; GENE-EXPRESSION ; SALIVARY-GLAND ; PROTEINS ; ALIGNMENT ; FLUID ; METAMORPHOSIS ; VISUALIZATION ; CONFORMATION
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	MDPI
WOS记录号	WOS:000533909300013
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/7663]
专题	深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
通讯作者	He, Lisheng
作者单位	1.City Univ Hong Kong, Dept Comp Sci, Hong Kong 999077, Peoples R China 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 3.Chinese Acad Sci, Ctr Human Tissues & Organs Degenerat, Inst Biomed & Biotechnol, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China 4.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Div Life Sci, Hong Kong 999077, Peoples R China 5.Hong Kong Univ Sci & Technol, Hong Kong Branch, Southern Marine Sci & Engn Guangdong Lab Guangzho, Hong Kong 999077, Peoples R China
推荐引用方式 GB/T 7714	Yan, Guoyong,Sun, Jin,Wang, Zishuai,et al. Insights into the Synthesis, Secretion and Curing of Barnacle Cyprid Adhesive via Transcriptomic and Proteomic Analyses of the Cement Gland[J]. MARINE DRUGS,2020,18(4):19.
APA	Yan, Guoyong,Sun, Jin,Wang, Zishuai,Qian, Pei-Yuan,&He, Lisheng.(2020).Insights into the Synthesis, Secretion and Curing of Barnacle Cyprid Adhesive via Transcriptomic and Proteomic Analyses of the Cement Gland.MARINE DRUGS,18(4),19.
MLA	Yan, Guoyong,et al."Insights into the Synthesis, Secretion and Curing of Barnacle Cyprid Adhesive via Transcriptomic and Proteomic Analyses of the Cement Gland".MARINE DRUGS 18.4(2020):19.